Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD1d Alexa Fluor<sup>®</sup> 647

Anti-Hu CD1d Alexa Fluor^® 647

Regulatory status

RUO

Antigen

CD1d

Clone

51.1

Format

Alexa Fluor^® 647

Reactivity

Human, Non-human primates

Application

WB, FC (QC tested), IHC(F)

Excitation laser

red (633 nm)

Variant

100 tests

A6-924-T100

In stock

242.00 USD

Variant

100 tests

A6-924-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody 51.1 recognizes an extracellular epitope of CD1d, a 38 kDa transmembrane glycoprotein expressed mainly on cortical thymocytes, marginal zone B cells and other antigen presenting cells, but also i e.g. hepatitis C virus-infected livers.

Application

WB, FC (QC tested), IHC(F)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human, Non-human primates

Immunogen

human CD1d

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Antigen description

CD1d belongs to CD1 family of transmembrane glycoproteins, associated with beta2 microglobulin, similarly to MHC I molecules. Unlike other CD1 family members, however, CD1d can be also expressed in a non-glycosylated form, which is not associated with beta2 microglobulin. Hence it is not certain how much CD1d plays a role in the presentation of microbial lipid antigens during infection. On the other hand, it is expressed on various antigen presenting cell types. Besides it, CD1d+ thymocytes are involved in the positive selection of sublineage of NKT cells.

Entrez Gene ID 912

UniProt ID P15813

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD1d (51.1) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD1d positive lymphocytes (red-filled) from CD1d negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD1d (51.1) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Kim HS, Garcia J, Exley M, Johnson KW, Balk SP, Blumberg RS: Biochemical characterization of CD1d expression in the absence of beta2-microglobulin. J Biol Chem. 1999 Apr 2;274(14):9289-95.
PubMed

Durante-Mangoni E, Wang RJ, Shaulov A, He Q, Nasser I, Afdhal N, Koziel MJ, Exley MA: Hepatic CD1d expression in hepatitis C virus infection and recognition by resident proinflammatory CD1d-reactive T cells. J Immunol. 2004 Aug 1;173(3):2159-66.
PubMed