Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD1c PE

Anti-Hu CD1c PE

Regulatory status

RUO

Antigen

CD1c

Clone

L161

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-752-T100

In stock

242.00 USD

25 tests

1P-752-T025

Delivery 1 week

121.00 USD

Variant

100 tests

1P-752-T100

In stock

242.00 USD

25 tests

1P-752-T025

Delivery 1 week

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody L161 recognizes an extracellular epitope of CD1c, (R7), a 43 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells, B and some T cells.

Workshop

HLDA V

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

human thymocytes

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

R7, BDCA1

Antigen description

CD1c (also known as R7 or BDCA1) together with CD1a and b, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. CD1c is unique in its ability to present e.g. mycobacterial phosphoketides and polyisoprenoids. CD1c is the only CD1 isoform that has been shown to interact both with alpha/beta and gamma/delta T cells.

Entrez Gene ID 911

UniProt ID P29017

Anti-human CD1c PE antibody (clone L161) works in flow cytometry application.

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Mouse monoclonal anti-human CD1c PE antibody (clone L161) was used in concentration 1 µg/ml in stained blood sample (2 x 10⁶ cells).

Expression profiling on peripheral blood subsets using Anti-human CD1c PE antibody (clone L161). Adaptive panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 106 cells) was added to the mixture of anti-human CD1c PE antibody (clone L161, 1 µg/ml in stained blood sample) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Adaptive) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Expression profiling on peripheral blood subsets using Anti-human CD1c PE antibody (clone L161). Innate panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 106 cells) was added to the mixture of anti-human CD1c PE antibody (clone L161, 1 µg/ml in stained blood sample) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Innate) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Product specific references:

del C Salamone M, Mendiguren AK, Salamone GV, Fainboim L: Membrane trafficking of CD1c on activated T cells. J Leukoc Biol. 2001 Oct;70(4):567-77.
PubMed

Xu C, de Vries R, Visser L, Diepstra A, Gadola SD, Poppema S, van den Berg A: Expression of CD1d and presence of invariant NKT cells in classical Hodgkin lymphoma. Am J Hematol. 2010 Jul;85(7):539-41. doi: 10.1002/ajh.21743.
PubMed

Todate A, Chida K, Suda T, Imokawa S, Sato J, Ide K, Tsuchiya T, Inui N, Nakamura Y, Asada K, Hayakawa H, Nakamura H: Increased numbers of dendritic cells in the bronchiolar tissues of diffuse panbronchiolitis. Am J Respir Crit Care Med. 2000 Jul;162(1):148-53.
PubMed

Scharf L, Li NS, Hawk AJ, Garzón D, Zhang T, Fox LM, Kazen AR, Shah S, Haddadian EJ, Gumperz JE, Saghatelian A, Faraldo-Gómez JD, Meredith SC, Piccirilli JA, Adams EJ: The 2.5 Å structure of CD1c in complex with a mycobacterial lipid reveals an open groove ideally suited for diverse antigen presentation. Immunity. 2010 Dec 14;33(6):853-62.
PubMed

Gutzeit C, Raftery MJ, Peiser M, Tischer KB, Ulrich M, Eberhardt M, Stockfleth E, Giese T, Sauerbrei A, Morita CT, Schönrich G: Identification of an important immunological difference between virulent varicella-zoster virus and its avirulent vaccine: viral disruption of dendritic cell instruction. J Immunol. 2010 Jul 1;185(1):488-97.
PubMed

Briken V, Jackman RM, Watts GF, Rogers RA, Porcelli SA: Human CD1b and CD1c isoforms survey different intracellular compartments for the presentation of microbial lipid antigens. J Exp Med. 2000 Jul 17;192(2):281-8.
PubMed

del C Salamone M, Mendiguren AK, Salamone GV, Fainboim L: Membrane trafficking of CD1c on activated T cells. J Leukoc Biol. 2001 Oct;70(4):567-77.
PubMed

Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed