Anti-Hu CD1c APC

Anti-Hu CD1c APC
Regulatory status
RUO
Antigen
CD1c
Clone
L161
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-752-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody L161 recognizes an extracellular epitope of CD1c, (R7), a 43 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells, B and some T cells.
Workshop
HLDA X
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
human thymocytes
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
R7, BDCA1
Antigen description
CD1c (also known as R7 or BDCA1) together with CD1a and b, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. CD1c is unique in its ability to present e.g. mycobacterial phosphoketides and polyisoprenoids. CD1c is the only CD1 isoform that has been shown to interact both with alpha/beta and gamma/delta T cells.
Entrez Gene ID 911
UniProt ID P29017
1A-752_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD1c (L161) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1A-752_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD1c (L161) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (LT19) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) antibody.
1A-752_FC_Histogram
Separation of human CD1c positive CD19 positive B cells (red-filled) from human CD1c negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD1c (L161) APC (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

del C Salamone M, Mendiguren AK, Salamone GV, Fainboim L: Membrane trafficking of CD1c on activated T cells. J Leukoc Biol. 2001 Oct;70(4):567-77.
PubMed
Xu C, de Vries R, Visser L, Diepstra A, Gadola SD, Poppema S, van den Berg A: Expression of CD1d and presence of invariant NKT cells in classical Hodgkin lymphoma. Am J Hematol. 2010 Jul;85(7):539-41. doi: 10.1002/ajh.21743.
PubMed
Todate A, Chida K, Suda T, Imokawa S, Sato J, Ide K, Tsuchiya T, Inui N, Nakamura Y, Asada K, Hayakawa H, Nakamura H: Increased numbers of dendritic cells in the bronchiolar tissues of diffuse panbronchiolitis. Am J Respir Crit Care Med. 2000 Jul;162(1):148-53.
PubMed
Scharf L, Li NS, Hawk AJ, Garzón D, Zhang T, Fox LM, Kazen AR, Shah S, Haddadian EJ, Gumperz JE, Saghatelian A, Faraldo-Gómez JD, Meredith SC, Piccirilli JA, Adams EJ: The 2.5 Å structure of CD1c in complex with a mycobacterial lipid reveals an open groove ideally suited for diverse antigen presentation. Immunity. 2010 Dec 14;33(6):853-62.
PubMed
Gutzeit C, Raftery MJ, Peiser M, Tischer KB, Ulrich M, Eberhardt M, Stockfleth E, Giese T, Sauerbrei A, Morita CT, Schönrich G: Identification of an important immunological difference between virulent varicella-zoster virus and its avirulent vaccine: viral disruption of dendritic cell instruction. J Immunol. 2010 Jul 1;185(1):488-97.
PubMed
Briken V, Jackman RM, Watts GF, Rogers RA, Porcelli SA: Human CD1b and CD1c isoforms survey different intracellular compartments for the presentation of microbial lipid antigens. J Exp Med. 2000 Jul 17;192(2):281-8.
PubMed
del C Salamone M, Mendiguren AK, Salamone GV, Fainboim L: Membrane trafficking of CD1c on activated T cells. J Leukoc Biol. 2001 Oct;70(4):567-77.
PubMed
Variant
100 tests
1A-752-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

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