Anti-Hu CD1b APC

Anti-Hu CD1b APC
Regulatory status
RUO
Antigen
CD1b
Clone
SN13
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-761-T100
In stock
242.00 USD
Variant
100 tests
1A-761-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody SN13 (also known as K5-1B8) recognizes an extracellular epitope of CD1b, a 44 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells.
Workshop
HCDM listed
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
A cell membrane antigen preparation that was isolated from normal human thymocytes
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
R1
Antigen description
CD1b (also known as R1) together with CD1a and c, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. Besides non-peptide glycolipid antigen presentation to CD1-restricted T cells, CD1b has been implicated in thymocyte development.
Entrez Gene ID 910
UniProt ID P29016
1A-761_FC_Profil Značení
Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1b (SN13) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
1A-761_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells using anti-human CD1b (SN13) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD11c (BU15) PE antibody (20 μl reagent per milion cells in 100 μl of cell suspension).
1A-761_FC_Histogram
Separation of human CD11c positive CD1b positive cells (red-filled) from CD11c negative CD1b negative cells (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1b (SN13) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Product specific references:

Hayes SM, Knight KL: Group 1 CD1 genes in rabbit. J Immunol. 2001 Jan 1;166(1):403-10.
PubMed
Tentori L, Graziani G, Porcelli SA, Sugita M, Brenner MB, Madaio R, Bonmassar E, Giuliani A, Aquino A: Rifampin increases cytokine-induced expression of the CD1b molecule in human peripheral blood monocytes. Antimicrob Agents Chemother. 1998 Mar;42(3):550-4.
PubMed
Giuliani A, Prete SP, Graziani G, Aquino A, Balduzzi A, Sugita M, Brenner MB, Iona E, Fattorini L, Orefici G, Porcelli SA, Bonmassar E: Influence of Mycobacterium bovis bacillus Calmette Guérin on in vitro induction of CD1 molecules in human adherent mononuclear cells. Infect Immun. 2001 Dec;69(12):7461-70.
PubMed
Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
Variant
100 tests
1A-761-T100
In stock
242.00 USD
Variant
100 tests
1A-761-T100
In stock
242.00 USD

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