Anti-Hu CD1a FITC

Anti-Hu CD1a FITC
Regulatory status
RUO
Antigen
CD1a
Clone
SK9
Format
FITC
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1F-174-T100
In stock
198.00 USD

25 tests
1F-174-T025
In stock
99.00 USD
Variant
100 tests
1F-174-T100
In stock
198.00 USD

25 tests
1F-174-T025
In stock
99.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2b kappa
Specificity
The mouse monoclonal antibody SK9 recognizes an extracellular epitope of CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human thymocytes
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
T6, Leu-6, HTA1, FCB6
Antigen description
CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.
Entrez Gene ID 909
UniProt ID P06126
1F-174_FC_Profil Značení
Flow cytometry surface staining pattern of human stimulated (GM-CSF and IL-4) peripheral blood mononuclear cells stained using anti-human CD1a (SK9) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).
1F-174_FC_Dot-plot
Flow cytometry multicolor surface staining of human stimulated (GM-CSF and IL-4) peripheral blood mononuclear cells stained using anti-human CD1a (SK9) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD11c (BU15) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
1F-174_FC_Histogram
Separation of human CD1a positive CD11c positive cells (red-filled) from CD1a negative CD11c negative cells (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF and IL-4) peripheral blood mononuclear cells stained using anti-human CD1a (SK9) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

Product specific references:

Kader M, Smith AP, Guiducci C, Wonderlich ER, Normolle D, Watkins SC, Barrat FJ, Barratt-Boyes SM: Blocking TLR7- and TLR9-mediated IFN-α production by plasmacytoid dendritic cells does not diminish immune activation in early SIV infection. PLoS Pathog. 2013;9(7):e1003530.
PubMed
Awasthi S, Wolf R, White G: Ontogeny and phagocytic function of baboon lung dendritic cells. Immunol Cell Biol. 2009 Jul;87(5):419-27.
PubMed
Tenca C, Merlo A, Zarcone D, Saverino D, Bruno S, De Santanna A, Ramarli D, Fabbi M, Pesce C, Deaglio S, Ciccone E, Malavasi F, Grossi CE: Death of T cell precursors in the human thymus: a role for CD38. Int Immunol. 2003 Sep;15(9):1105-16.
PubMed
Brown KN, Trichel A, Barratt-Boyes SM: Parallel loss of myeloid and plasmacytoid dendritic cells from blood and lymphoid tissue in simian AIDS. J Immunol. 2007 Jun 1;178(11):6958-67.
PubMed
Zimmer MI, Larregina AT, Castillo CM, Capuano S 3rd, Falo LD Jr, Murphey-Corb M, Reinhart TA, Barratt-Boyes SM: Disrupted homeostasis of Langerhans cells and interdigitating dendritic cells in monkeys with AIDS. Blood. 2002 Apr 15;99(8):2859-68.
PubMed
Wood GS, Burns BF, Dorfman RF, Warnke RA: The immunohistology of non-T cells in the acquired immunodeficiency syndrome. Am J Pathol. 1985 Sep;120(3):371-9.
PubMed
Variant
100 tests
1F-174-T100
In stock
198.00 USD

25 tests
1F-174-T025
In stock
99.00 USD
Variant
100 tests
1F-174-T100
In stock
198.00 USD

25 tests
1F-174-T025
In stock
99.00 USD

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