Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD1a APC

Anti-Hu CD1a APC

Regulatory status

RUO

Antigen

CD1a

Clone

HI149

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-364-T100

In stock

242.00 USD

25 tests

1A-364-T025

In stock

121.00 USD

Variant

100 tests

1A-364-T100

In stock

242.00 USD

25 tests

1A-364-T025

In stock

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody HI149 reacts with an extracellular epitope of CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas. The antibody does not react with peripheral blood T and B lymphocytes, monocytes, granulocytes, platelets and erythrocytes.

Workshop

HLDA V: WS Code CD01.01

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Human thymocytes

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

T6, Leu-6, HTA1, FCB6

Antigen description

CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.

Entrez Gene ID 909

UniProt ID P06126

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1a (HI149) APC antibody (concentration in sample 0.5 μg/ml).

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1a (HI149) APC antibody (concentration in sample 0.5 μg/ml) and anti-human CD11c (BU15) PE antibody (20 μl reagent per milion cells in 100 μl of cell suspension).

Separation of CD11c positive cells stained using CD1a (HI149) APC antibody (concentration in sample 0.5 μg/ml, red-filled) from CD11c positive cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 0.5 μg/ml, same as CD1a APC concentration, black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells.

Product specific references:

Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 27;9:852.
PubMed

Fromm JR, Thomas A, Wood BL: Characterization and purification of neoplastic cells of nodular lymphocyte predominant hodgkin lymphoma from lymph nodes by flow cytometry and flow cytometric cell sorting. Am J Pathol. 2017 Feb;187(2):304-317.
PubMed