Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD1a APC

Anti-Hu CD1a APC

Regulatory status

RUO

Antigen

CD1a

Clone

SK9

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-174-T100

In stock

248.60 USD

25 tests

1A-174-T025

In stock

124.30 USD

Variant

100 tests

1A-174-T100

In stock

248.60 USD

25 tests

1A-174-T025

In stock

124.30 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody SK9 recognizes an extracellular epitope of CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Human thymocytes

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

T6, Leu-6, HTA1, FCB6

Antigen description

CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.

Entrez Gene ID 909

UniProt ID P06126

Flow cytometry surface staining pattern of human GM-CSF and IL-4 stimulated peripheral blood mononuclear cells stained using anti-human CD1a (SK9) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD1a positive cells (red-filled) from cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 5 μg/ml, same as CD11a APC concentration, black-dashed) in flow cytometry analysis (surface staining) of human human GM-CSF and IL-4 stimulated peripheral blood mononuclear cells stained using anti-human CD1a (SK9) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of MOLT-4 cells stained using anti-human CD1a (SK9) APC antibody (concentration in sample 4 μg/m, red-filled) from MOLT-4 cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 4 μg/ml, same as CD1a APC concentration, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Kader M, Smith AP, Guiducci C, Wonderlich ER, Normolle D, Watkins SC, Barrat FJ, Barratt-Boyes SM: Blocking TLR7- and TLR9-mediated IFN-α production by plasmacytoid dendritic cells does not diminish immune activation in early SIV infection. PLoS Pathog. 2013;9(7):e1003530.
PubMed

Awasthi S, Wolf R, White G: Ontogeny and phagocytic function of baboon lung dendritic cells. Immunol Cell Biol. 2009 Jul;87(5):419-27.
PubMed

Tenca C, Merlo A, Zarcone D, Saverino D, Bruno S, De Santanna A, Ramarli D, Fabbi M, Pesce C, Deaglio S, Ciccone E, Malavasi F, Grossi CE: Death of T cell precursors in the human thymus: a role for CD38. Int Immunol. 2003 Sep;15(9):1105-16.
PubMed

Brown KN, Trichel A, Barratt-Boyes SM: Parallel loss of myeloid and plasmacytoid dendritic cells from blood and lymphoid tissue in simian AIDS. J Immunol. 2007 Jun 1;178(11):6958-67.
PubMed

Zimmer MI, Larregina AT, Castillo CM, Capuano S 3rd, Falo LD Jr, Murphey-Corb M, Reinhart TA, Barratt-Boyes SM: Disrupted homeostasis of Langerhans cells and interdigitating dendritic cells in monkeys with AIDS. Blood. 2002 Apr 15;99(8):2859-68.
PubMed

Wood GS, Burns BF, Dorfman RF, Warnke RA: The immunohistology of non-T cells in the acquired immunodeficiency syndrome. Am J Pathol. 1985 Sep;120(3):371-9.
PubMed