Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD199 PE-Cy™7

Anti-Hu CD199 PE-Cy™7

Regulatory status

RUO

Antigen

CD199

Clone

C9Mab-1

Format

PE-Cy™7

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

T7-902-T100

In stock

282.70 USD

Variant

100 tests

T7-902-T100

In stock

282.70 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody C9Mab-1 recognizes an extracellular epitope of CD199 (Chemokine Receptor 9), a 7-transmembrane chemokine receptor.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

CD199 transfected CHO cells

Preparation

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

GPR-9-6, GPR28, CCR9

Antigen description

CD199 (CCR9) is a G-protein-coupled 7 TM chemokine receptor for TECK (SCYA25) chemokine. It is expressed strongly in thymus, at lower level in bone marrow and spleen, as well as on a subset of memory T cells specialized for mucosal homing. CD199 appears to confer homing properties to the small intestine on memory T cells. On the other hand it functions as a coreceptor for HIV-1.

Entrez Gene ID 10803

UniProt ID P51686

Separation of MOLT-4 cells stained using anti-human CD199 (C9Mab-1) PE-Cy™7 antibody (concentration in sample 5 μg/ml, red-filled) from MOLT-4 cells stained using mouse IgG1 isotype control (MOPC-21) PE-Cy™7 antibody (concentration in sample 5 μg/ml, same as CD199 PE-Cy™7 concentration, black-dashed) in flow cytometry analysis (surface staining).