Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD195 PE

Anti-Hu CD195 PE

Regulatory status

RUO

Antigen

CD195

Clone

T21/8

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-707-T100

In stock

248.60 USD

25 tests

1P-707-T025

Delivery 1 week

124.30 USD

Variant

100 tests

1P-707-T100

In stock

248.60 USD

25 tests

1P-707-T025

Delivery 1 week

124.30 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody T21/8 recognizes an extracellular epitope on the N-teminus of CD195, an approximately 45 kDa G-protein coupled receptor 1 family protein expressed on resting T cells, monocytes, macrophages, and immature dendritic cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

CCR5 peptide (Met1-Lys22) KLH conjugate

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CKR5, CCR5, CCCKR5, CMKBR5, IDDM22, CC-CKR-5

Antigen description

CD195 / CCR5 (also known as CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 alpha, MIP-1 beta, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 / CCR5 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120.

Entrez Gene ID 1234

UniProt ID P51681

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD195 (T21/8) PE antibody (concentration in sample 1 μg/ml).

Flow cytometry multicolor surface staining pattern of human lymfocytes stained using anti-human CD4 (MEM-241) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD195 (T21/8) PE antibody (concentration in sample 1 μg/ml).

Separation of human CD4 negative CD195 positive cells (red-filled) from CD4 positive CD195 negative Th cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD195 (T21/8) PE antibody (concentration in sample 1 μg/ml).

Anti-Hu CD195 PE antibody (clone T21/8) specificity verification by flow cytometry

Anti-Hu CD195 PE (concentration in sample 5 μg/ml, red-filled histogram) binds specifically to surface CD195 in transfected cells (upper panel), but not to the parent HEK-293 WT cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE (MOPC-21) under same conditions (concentration in sample 5 μg/ml, black-dashed histogram).

Product specific references:

Pollok-Kopp B, Schwarze K, Baradari VK, Oppermann M: Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies. J Biol Chem. 2003 Jan 24;278(4):2190-8.
PubMed

Hüttenrauch F, Pollok-Kopp B, Oppermann M: G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers. J Biol Chem. 2005 Nov 11;280(45):37503-15.
PubMed

Monde K, Maeda Y, Tanaka Y, Harada S, Yusa K: Gp120 V3-dependent impairment of R5 HIV-1 infectivity due to virion-incorporated CCR5. J Biol Chem. 2007 Dec 21;282(51):36923-32.
PubMed

Mascalchi P, Lamort AS, Salomé L, Dumas F: Single Particle Tracking reveals two distinct environments for CD4 receptors at the surface of living T lymphocytes. Biochem Biophys Res Commun. 2012 Jan 6;417(1):409-13
PubMed