Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD195 APC

Anti-Hu CD195 APC

Regulatory status

RUO

Antigen

CD195

Clone

T21/8

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-707-T100

Ask for delivery term HERE

248.60 USD

25 tests

1A-707-T025

Delivery 1 week

124.30 USD

Variant

100 tests

1A-707-T100

Ask for delivery term HERE

248.60 USD

25 tests

1A-707-T025

Delivery 1 week

124.30 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody T21/8 recognizes an extracellular epitope on the N-teminus of CD195, an approximately 45 kDa G-protein coupled receptor 1 family protein expressed on resting T cells, monocytes, macrophages, and immature dendritic cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

CCR5 peptide (Met1-Lys22) KLH conjugate

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CKR5, CCR5, CCCKR5, CMKBR5, IDDM22, CC-CKR-5

Antigen description

CD195 / CCR5 (also known as CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 alpha, MIP-1 beta, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 / CCR5 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120.

Entrez Gene ID 1234

UniProt ID P51681

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD195 (T21/8) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD4 (MEM-241) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD195 (T21/8) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD195 positive CD4 negative lymphocytes (red-filled) from CD195 negative CD4 positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD195 (T21/8) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Pollok-Kopp B, Schwarze K, Baradari VK, Oppermann M: Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies. J Biol Chem. 2003 Jan 24;278(4):2190-8.
PubMed

Hüttenrauch F, Pollok-Kopp B, Oppermann M: G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers. J Biol Chem. 2005 Nov 11;280(45):37503-15.
PubMed

Monde K, Maeda Y, Tanaka Y, Harada S, Yusa K: Gp120 V3-dependent impairment of R5 HIV-1 infectivity due to virion-incorporated CCR5. J Biol Chem. 2007 Dec 21;282(51):36923-32.
PubMed

Mascalchi P, Lamort AS, Salomé L, Dumas F: Single Particle Tracking reveals two distinct environments for CD4 receptors at the surface of living T lymphocytes. Biochem Biophys Res Commun. 2012 Jan 6;417(1):409-13
PubMed