Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD194 APC

Anti-Hu CD194 APC

Regulatory status

RUO

Antigen

CD194

Clone

rHIQB002

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

test1 True

Variant

100 tests

1A-961-T100

In stock

248.60 USD

Variant

100 tests

1A-961-T100

In stock

248.60 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG1

Specificity

The recombinant mouse monoclonal antibody rHIQB002 recognizes an extracellular epitope of human chemokine receptor CD194 (CCR4).

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CCR4, CC-CKR-4, CKR4, CMKBR4, ChemR13, HGCN

Antigen description

CD194 is a G-protein-coupled seven membrane-spanning transmembrane protein serving as a high affinity receptor for C-C type chemokines TARC (CCL17) and MDC (CCL22), and as a coreceptor for HIV. It is expressed by all CLA+ T cells regardless of their Th1 or Th2 phenotype, on activated Th2, Treg, and activated NK cells, basophils and macrophages. Blocking CD194 function can be used for treatment of inflammation.

Entrez Gene ID 1233

UniProt ID P51679

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD194 (rHIQB002) APC antibody (concentration in sample 0.19 μg/ml).

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD194 (rHIQB002) APC antibody (concentration in sample 0.19 μg/ml).

Separation of human CD3 positive CD194 positive T cells (red-filled) from CD3 negative CD194 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD194 (rHIQB002) APC antibody (concentration in sample 0.19 μg/ml).