Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD193 Pacific Blue™

Anti-Hu CD193 Pacific Blue™

Regulatory status

RUO

Antigen

CD193

Clone

5E8

Format

Pacific Blue™

Reactivity

Human

Application

FC (QC tested)

Excitation laser

violet (405 nm)

Variant

100 tests

PB-161-T100

In stock

242.00 USD

Variant

100 tests

PB-161-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody 5E8 recognizes an extracellular epitope of CD193 (chemokine receptor 3), an approximately 41 kDa protein expressed above all in eosinophils and basophils.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

human CD193 transfectants

Preparation

Purified antibody is conjugated with Pacific Blue™ NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

CCR3, CKR3, CMKBR3, CC-CKR-3

Antigen description

CD193 / CCR3 is a G-protein coupled receptor for several chemokines, namely CCL11 (eotaxin), CCL26 (eotaxin-3), CCL7 (MCP-4), or CCL5 (RANTES). It is highly expressed on eosinophils and basophils, and is also detected in TH1 and TH2 cells, as well as in airway epithelial cells. CD193 is the key eosinophil chemokine receptor responsible for regulation of eosinophil migration and function. This receptor may contribute to the accumulation and activation of eosinophils and other inflammatory cells in the allergic airway. It is also known to be an entry co-receptor for HIV-1.

Entrez Gene ID 1232

UniProt ID P51677

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD193 (5E8) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human basophil granulocytes (red-filled) from CD193 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD193 (5E8) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Morshed M, Hlushchuk R, Simon D, Walls AF, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon HU, Yousefi S: NADPH oxidase-independent formation of extracellular DNA traps by basophils. J Immunol. 2014 Jun 1;192(11):5314-23.
PubMed