Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD183 FITC

Anti-Hu CD183 FITC

Regulatory status

RUO

Antigen

CD183

Clone

rHIQB005

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

test1 True

Variant

100 tests

1F-960-T100

In stock

203.50 USD

Variant

100 tests

1F-960-T100

In stock

203.50 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG1

Specificity

The recombinant mouse monoclonal antibody rHIQB005 recognizes an extracellular epitope on human chemokine receptor CD184.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CXCR3, fusin, CKR-L2, CMKAR3, GPR9, IP10-R, MIG-R

Antigen description

CD184 is a 7-transmembrane G-protein coupled receptor for the C-X-C chemokines SDF-1 (CXCL12), MIG (CXCL9) and IP10 (CXCL10). Alternatively spliced variant CD184B serves as the high affinity receptor for PF4 (CXCL4). CD184 is expressed in hematopoietic cells, vascular endothelium, and neural tissue. It plays important roles in endothelial vascularization and central nervous system development. It can also serve as a primary receptor or a coreceptor for some HIV isolates. CD184 signaling leads to integrin activation, cytoskeletal changes and chemotactic migration.

Entrez Gene ID 2833

UniProt ID P49682

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD183 (rHIQB005) FITC antibody (concentration in sample 1.67 μg/ml).

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD183 (rHIQB005) FITC antibody (concentration in sample 1.67 μg/ml).

Separation of human CD3 positive CD183 positive T cells (red-filled) from CD3 negative CD183 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD183 (rHIQB005) FITC antibody (concentration in sample 1.67 μg/ml).