Anti-Hu CD180 Purified

Anti-Hu CD180 Purified
Regulatory status
RUO
Antigen
CD180
Clone
G28-8
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-637-C100
In stock
187.00 USD

0.025 mg
11-637-C025
Delivery 1 week
93.50 USD
Variant
0.1 mg
11-637-C100
In stock
187.00 USD

0.025 mg
11-637-C025
Delivery 1 week
93.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody G28-8 recognizes an extracellular epitope of CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.
Workshop
HLDA workshop
Application details
Flow cytometry: Recommended dilution: 2 μg/ml.
Reactivity
Human
Immunogen
Human tonsillar B cells
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
LY64, Ly78, RP105, Bgp95
Antigen description
CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1, and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size.
Entrez Gene ID 4064
UniProt ID Q99467
11-637_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD180 (G28-8) purified antibody (concentration in sample 6 μg/ml) GAM APC.
11-637_FC_Histogram
Separation of human CD180 positive lymphocytes (red-filled) from CD180 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD180 (G28-8) purified antibody (concentration in sample 6 μg/ml) GAM APC.

General references:

Kikuchi Y, Koarada S, Tada Y, Ushiyama O, Morito F, Suzuki N, Ohta A, Horiuchi T, Miyake K, Nagasawa K: Difference in B cell activation between dermatomyositis and polymyositis: analysis of the expression of RP105 on peripheral blood B cells. Ann Rheum Dis. 2001 Dec;60(12):1137-40.
PubMed
Divanovic S, Trompette A, Petiniot LK, Allen JL, Flick LM, Belkaid Y, Madan R, Haky JJ, Karp CL: Regulation of TLR4 signaling and the host interface with pathogens and danger: the role of RP105. J Leukoc Biol. 2007 Aug;82(2):265-71.
PubMed
Yazawa N, Fujimoto M, Sato S, Miyake K, Asano N, Nagai Y, Takeuchi O, Takeda K, Okochi H, Akira S, Tedder TF, Tamaki K: CD19 regulates innate immunity by the toll-like receptor RP105 signaling in B lymphocytes. Blood. 2003 Aug 15;102(4):1374-80.
PubMed
Divanovic S, Trompette A, Atabani SF, Madan R, Golenbock DT, Visintin A, Finberg RW, Tarakhovsky A, Vogel SN, Belkaid Y, Kurt-Jones EA, Karp CL: Negative regulation of Toll-like receptor 4 signaling by the Toll-like receptor homolog RP105. Nat Immunol. 2005 Jun;6(6):571-8.
PubMed

Product specific references:

Valentine MA, Clark EA, Shu GL, Norris NA, Ledbetter JA: Antibody to a novel 95-kDa surface glycoprotein on human B cells induces calcium mobilization and B cell activation. J Immunol. 1988 Jun 15;140(12):4071-8.
PubMed
Clark EA, Shu GL, Lüscher B, Draves KE, Banchereau J, Ledbetter JA, Valentine MA: Activation of human B cells. Comparison of the signal transduced by IL-4 to four different competence signals. J Immunol. 1989 Dec 15;143(12):3873-80.
PubMed
Olson NE, Graves JD, Shu GL, Ryan EJ, Clark EA: Caspase activity is required for stimulated B lymphocytes to enter the cell cycle. J Immunol. 2003 Jun 15;170(12):6065-72.
PubMed
Variant
0.1 mg
11-637-C100
In stock
187.00 USD

0.025 mg
11-637-C025
Delivery 1 week
93.50 USD
Variant
0.1 mg
11-637-C100
In stock
187.00 USD

0.025 mg
11-637-C025
Delivery 1 week
93.50 USD

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