Anti-Hu CD178 Purified Low Endotoxin

Anti-Hu CD178 Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD178
Clone
NOK-1
Format
Purified Low Endotoxin
Reactivity
Human
Variant
0.1 mg
12-754-C100
In stock
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody NOK-1 recognizes an extracellular epitope of CD178 / Fas-L, an approximately 40 kDa transmembrane glycoprotein expressed on neutrophils, monocytes, and activated T and NK cells.
Workshop
HLDA VII: 70322
Application details
Functional application: Blocking.
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Human
Immunogen
L5178Y mouse T lymphoma cells expressing recombinant human CD178
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
APTL, FASL, FAS-ligand, CD95L, TNFSF6, TNLG1A
Antigen description
CD178 / Fas-L (Fas ligand, CD95L), a member of TNF family transmembrane glycoproteins, is responsible for induction of apoptosis in cells containing its receptor CD95 / Fas. The CD178-mediated apoptosis pathway has been implicated in peripheral tolerance, tissue pathology, and maintenance of the immune privileged sites. Defects in this interaction may be related to some cases of systemic lupus erythematosus (SLE). CD178 was also described as a co-stimulatory receptor for T-cell activation in mice in vivo.
Entrez Gene ID 356
UniProt ID P48023
12-754_FC_Profil Značení
Flow cytometry surface staining pattern of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (low endotoxin, concentration in sample 9 μg/ml) GAM APC.
12-754_FC_Histogram
Separation of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (low endotoxin, concentration in sample 9 μg/ml, GAM APC, red-filled) from FasL transfected L5178Y cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Ji J, Chen JJ, Braciale VL, Cloyd MW: Apoptosis induced in HIV-1-exposed, resting CD4+ T cells subsequent to signaling through homing receptors is Fas/Fas ligand-mediated. J Leukoc Biol. 2007 Jan;81(1):297-305.
PubMed
Kayagaki N, Kawasaki A, Ebata T, Ohmoto H, Ikeda S, Inoue S, Yoshino K, Okumura K, Yagita H: Metalloproteinase-mediated release of human Fas ligand. J Exp Med. 1995 Dec 1;182(6):1777-83.
PubMed
Jodo S, Pidiyar VJ, Xiao S, Furusaki A, Sharma R, Koike T, Ju ST: Fas ligand (CD178) cytoplasmic tail is a positive regulator of Fas ligand-mediated cytotoxicity. J Immunol. 2005 Apr 15;174(8):4470-4.
PubMed
Ghadimi MP, Sanzenbacher R, Thiede B, Wenzel J, Jing Q, Plomann M, Borkhardt A, Kabelitz D, Janssen O: Identification of interaction partners of the cytosolic polyproline region of CD95 ligand (CD178). FEBS Lett. 2002 May 22;519(1-3):50-8.
PubMed
Chopin D, Barei-Moniri R, Maillé P, Le Frère-Belda MA, Muscatelli-Groux B, Merendino N, Lecerf L, Stoppacciaro A, Velotti F: Human urinary bladder transitional cell carcinomas acquire the functional Fas ligand during tumor progression. Am J Pathol. 2003 Apr;162(4):1139-49.
PubMed
Variant
0.1 mg
12-754-C100
In stock
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

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