Anti-Hu CD177 FITC

Anti-Hu CD177 FITC
Regulatory status
RUO
Antigen
CD177
Clone
MEM-166
Format
FITC
Reactivity
Non-human primates, Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1F-314-T100
In stock
180.00 USD

25 tests
1F-314-T025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.
Workshop
HLDA VI: WS Code M M17; HLDA VI: WS Code BP 309
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Non-human primates, Human
Immunogen
Human granulocytes
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
NB1, PRV1, HNA2A
Antigen description
CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).
Entrez Gene ID 57126
UniProt ID Q8N6Q3
1F-314_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD177 (MEM-166) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).
1F-314_FC_Histogram
Separation of human CD177 positive neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD177 (MEM-166) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).

General references:

Bauer S, Abdgawad M, Gunnarsson L, Segelmark M, Tapper H, Hellmark T: Proteinase 3 and CD177 are expressed on the plasma membrane of the same subset of neutrophils. J Leukoc Biol. 2007 Feb;81(2):458-64.
PubMed
Sachs UJ, Andrei-Selmer CL, Maniar A, Weiss T, Paddock C, Orlova VV, Choi EY, Newman PJ, Preissner KT, Chavakis T, Santoso S: The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31). J Biol Chem. 2007 Aug 10;282(32):23603-12.
PubMed
Mnjoyan Z, Li J, Afshar-Kharghan V: Expression of polycythemia rubra vera-1 decreases the dependency of cells on growth factors for proliferation. Haematologica. 2005 Mar;90(3):405-6.
PubMed
Stroncek DF, Caruccio L, Bettinotti M: CD177: A member of the Ly-6 gene superfamily involved with neutrophil proliferation and polycythemia vera. J Transl Med. 2004 Mar 29;2(1):8.
PubMed
Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002).
Variant
100 tests
1F-314-T100
In stock
180.00 USD

25 tests
1F-314-T025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD

Related products

-134%
Reg. statusCE IVD
ApplicationFC
100 ml
200.00 USD
-134%
Reg. statusCE IVD
ApplicationFC
100 ml
60.00 USD
-134%
Reg. statusRUO
ApplicationFC
100 ml
50.00 USD
-134%
CloneMOPC-21
Reg. statusRUO
ApplicationFC, FC(IC)
0.1 mg
150.00 USD