Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD177 Alexa Fluor<sup>®</sup> 647

Anti-Hu CD177 Alexa Fluor^® 647

Regulatory status

RUO

Antigen

CD177

Clone

MEM-166

Format

Alexa Fluor^® 647

Reactivity

Non-human primates, Human

Application

WB, FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

A6-314-T100

In stock

242.00 USD

25 tests

A6-314-T025

In stock

121.00 USD

Variant

100 tests

A6-314-T100

In stock

242.00 USD

25 tests

A6-314-T025

In stock

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.

Workshop

HLDA VI: WS Code M M17; HLDA VI: WS Code BP 309

Application

WB, FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Non-human primates, Human

Immunogen

Human granulocytes

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

NB1, PRV1, HNA2A

Antigen description

CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).

Entrez Gene ID 57126

UniProt ID Q8N6Q3

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD177 (MEM-166) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of CD177 positive neutrophil granulocytes (red-filled) from CD177 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD177 (MEM-166) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

General references:

Bauer S, Abdgawad M, Gunnarsson L, Segelmark M, Tapper H, Hellmark T: Proteinase 3 and CD177 are expressed on the plasma membrane of the same subset of neutrophils. J Leukoc Biol. 2007 Feb;81(2):458-64.
PubMed

Sachs UJ, Andrei-Selmer CL, Maniar A, Weiss T, Paddock C, Orlova VV, Choi EY, Newman PJ, Preissner KT, Chavakis T, Santoso S: The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31). J Biol Chem. 2007 Aug 10;282(32):23603-12.
PubMed

Mnjoyan Z, Li J, Afshar-Kharghan V: Expression of polycythemia rubra vera-1 decreases the dependency of cells on growth factors for proliferation. Haematologica. 2005 Mar;90(3):405-6.
PubMed

Stroncek DF, Caruccio L, Bettinotti M: CD177: A member of the Ly-6 gene superfamily involved with neutrophil proliferation and polycythemia vera. J Transl Med. 2004 Mar 29;2(1):8.
PubMed

Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002).