Anti-Hu CD177 Alexa Fluor® 647

Anti-Hu CD177 Alexa Fluor<sup>®</sup> 647
Regulatory status
RUO
Antigen
CD177
Clone
MEM-166
Format
Alexa Fluor® 647
Reactivity
Non-human primates, Human
Excitation laser
red (633 nm)
Variant
100 tests
A6-314-T100
In stock
242.00 USD

25 tests
A6-314-T025
In stock
121.00 USD
Variant
100 tests
A6-314-T100
In stock
242.00 USD

25 tests
A6-314-T025
In stock
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.
Workshop
HLDA VI: WS Code M M17; HLDA VI: WS Code BP 309
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Non-human primates, Human
Immunogen
Human granulocytes
Preparation
Purified antibody is conjugated with Alexa Fluor® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
NB1, PRV1, HNA2A
Antigen description
CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).
Entrez Gene ID 57126
UniProt ID Q8N6Q3
A6-314_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD177 (MEM-166) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).
A6-314_FC_Histogram
Separation of CD177 positive neutrophil granulocytes (red-filled) from CD177 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD177 (MEM-166) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

General references:

Bauer S, Abdgawad M, Gunnarsson L, Segelmark M, Tapper H, Hellmark T: Proteinase 3 and CD177 are expressed on the plasma membrane of the same subset of neutrophils. J Leukoc Biol. 2007 Feb;81(2):458-64.
PubMed
Sachs UJ, Andrei-Selmer CL, Maniar A, Weiss T, Paddock C, Orlova VV, Choi EY, Newman PJ, Preissner KT, Chavakis T, Santoso S: The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31). J Biol Chem. 2007 Aug 10;282(32):23603-12.
PubMed
Mnjoyan Z, Li J, Afshar-Kharghan V: Expression of polycythemia rubra vera-1 decreases the dependency of cells on growth factors for proliferation. Haematologica. 2005 Mar;90(3):405-6.
PubMed
Stroncek DF, Caruccio L, Bettinotti M: CD177: A member of the Ly-6 gene superfamily involved with neutrophil proliferation and polycythemia vera. J Transl Med. 2004 Mar 29;2(1):8.
PubMed
Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002).
Variant
100 tests
A6-314-T100
In stock
242.00 USD

25 tests
A6-314-T025
In stock
121.00 USD
Variant
100 tests
A6-314-T100
In stock
242.00 USD

25 tests
A6-314-T025
In stock
121.00 USD

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