Anti-Hu CD173 Purified

Anti-Hu CD173 Purified
Regulatory status
RUO
Antigen
CD173
Clone
MEM-195
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-921-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Description
Images
References
Isotype
Mouse IgM
Specificity
The antibody MEM-195 reacts with CD173 (H2), an extracellular saccharide antigen expressed mainly during early hematopoiesis; it is also expressed on endothelial cells.
Workshop
HLDA VII
Application
Application details
Flow cytometry: Recommended dilution: 2-5 μg/ml.
Reactivity
Human
Immunogen
Human thrombocytes
Concentration
1 mg/ml
Preparation
Purified by precipitation and chromatography
Formulation
Tris buffered saline (TBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Lewis Y, blood group H2 antigen, LeY
Antigen description
CD173 (blood group antigen H2) is a fucosylated saccharide (Fuc-alpha-1-2-Gal-beta-1-4-GlcNAc-beta) generated by beta-D-galactoside 2-alpha-L-fucosyltransferase (FUT1). CD173 belongs to markers of early hematopoiesis; it is expressed mainly on CD34-positive hematopoietic progenitor cells. CD173 is a precursor structure of CD174 (Lewis Y) and is also structurally related to CD15 (Lewis X). On endothelial cells CD173 and CD174 are concentrated on pseudopodial extensions responsible for initial contacts between endothelial cells.
11-921_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD173 (MEM-195) purified antibody (concentration in sample 5 μg/ml, GAM PE).
11-921_FC_Histogram
Separation of human CD173 positive erythrocytes (red-filled) from human CD173 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD173 (MEM-195) purified antibody (concentration in sample 5 μg/ml, GAM PE).

General references:

Moehler TM, Sauer S, Witzel M, Andrulis M, Garcia-Vallejo JJ, Grobholz R, Willhauck-Fleckenstein M, Greiner A, Goldschmidt H, Schwartz-Albiez R: Involvement of alpha 1-2-fucosyltransferase I (FUT1) and surface-expressed Lewis(y) (CD174) in first endothelial cell-cell contacts during angiogenesis. J Cell Physiol. 2008 Apr;215(1):27-36.
PubMed
Cao Y, Merling A, Karsten U, Schwartz-Albiez R: The fucosylated histo-blood group antigens H type 2 (blood group O, CD173) and Lewis Y (CD174) are expressed on CD34+ hematopoietic progenitors but absent on mature lymphocytes. Glycobiology. 2001 Aug;11(8):677-83.
PubMed
Variant
0.1 mg
11-921-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD