Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD173 Purified

Anti-Hu CD173 Purified

Regulatory status

RUO

Antigen

CD173

Clone

MEM-195

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-921-C100

Ask for delivery term HERE

154.00 USD

Variant

0.1 mg

11-921-C100

Ask for delivery term HERE

154.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The antibody MEM-195 reacts with CD173 (H2), an extracellular saccharide antigen expressed mainly during early hematopoiesis; it is also expressed on endothelial cells.

Workshop

HLDA VII

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 2-5 μg/ml.

Reactivity

Human

Immunogen

Human thrombocytes

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Lewis Y, blood group H2 antigen, LeY

Antigen description

CD173 (blood group antigen H2) is a fucosylated saccharide (Fuc-alpha-1-2-Gal-beta-1-4-GlcNAc-beta) generated by beta-D-galactoside 2-alpha-L-fucosyltransferase (FUT1). CD173 belongs to markers of early hematopoiesis; it is expressed mainly on CD34-positive hematopoietic progenitor cells. CD173 is a precursor structure of CD174 (Lewis Y) and is also structurally related to CD15 (Lewis X). On endothelial cells CD173 and CD174 are concentrated on pseudopodial extensions responsible for initial contacts between endothelial cells.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD173 (MEM-195) purified antibody (concentration in sample 5 μg/ml, GAM PE).

Separation of human CD173 positive erythrocytes (red-filled) from human CD173 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD173 (MEM-195) purified antibody (concentration in sample 5 μg/ml, GAM PE).

General references:

Moehler TM, Sauer S, Witzel M, Andrulis M, Garcia-Vallejo JJ, Grobholz R, Willhauck-Fleckenstein M, Greiner A, Goldschmidt H, Schwartz-Albiez R: Involvement of alpha 1-2-fucosyltransferase I (FUT1) and surface-expressed Lewis(y) (CD174) in first endothelial cell-cell contacts during angiogenesis. J Cell Physiol. 2008 Apr;215(1):27-36.
PubMed

Cao Y, Merling A, Karsten U, Schwartz-Albiez R: The fucosylated histo-blood group antigens H type 2 (blood group O, CD173) and Lewis Y (CD174) are expressed on CD34+ hematopoietic progenitors but absent on mature lymphocytes. Glycobiology. 2001 Aug;11(8):677-83.
PubMed