Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD172g PE

Anti-Hu CD172g PE

Regulatory status

RUO

Antigen

CD172g

Clone

OX-119

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-881-T100

In stock

242.00 USD

Variant

100 tests

1P-881-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody OX-119 recognizes an extracellular epitope on CD172g, an approximately 55 kDa transmembrane glycoprotein expressed on most T cells, as well as on NK cells and some B cell populations.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

recombinant human CD172g

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SIRP gamma, SIRP-B2

Antigen description

CD172g is a transmembrane glycoprotein, which may play a role in inter-T cellular signaling by binding CD47, and thus in influencing T cell behaviour. CD172g is expressed on mature thymocytes, CD4+ T cells, CD8+ T cells, NK cells, and some B cells. It is absent on myeloid cells. Engagement of CD172g by CD47 expressed on antigen presenting cells results in enhanced antigen-specific T cell proliferation and costimulates T cell activation.

Entrez Gene ID 55423

UniProt ID Q9P1W8

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD172g (OX-119) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD172g positive lymphocytes (red-filled) from CD172g negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD172g (OX-119) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Nettleship JE, Ren J, Rahman N, Berrow NS, Hatherley D, Barclay AN, Owens RJ: A pipeline for the production of antibody fragments for structural studies using transient expression in HEK 293T cells. Protein Expr Purif. 2008 Nov;62(1):83-9
PubMed

Brooke G, Holbrook JD, Brown MH, Barclay AN: Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family. J Immunol. 2004 Aug 15;173(4):2562-70
PubMed