Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD172b PE

Anti-Hu CD172b PE

Regulatory status

RUO

Antigen

CD172b

Clone

B4B6

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-134-T100

In stock

242.00 USD

25 tests

1P-134-T025

Delivery 1 week

121.00 USD

Variant

100 tests

1P-134-T100

In stock

242.00 USD

25 tests

1P-134-T025

Delivery 1 week

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody B4B6 recognizes an extracellular epitope of CD172b, an approximately 50 kDa transmembrane glycoprotein expressed on myeloid cells.

Workshop

HLDA VIII

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

NIH-3T3 cells transfected with human CD172b

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SIRPB1

Antigen description

CD172b, the signal-regulatory protein beta (SIRP beta) is a disulfide-linked homodimer expressed on myeloid cells including monocytes and dendritic cells. Similarly to CD172a, it serves as a negative regulator of tyrosine kinase-coupled signaling processes. Unlike CD172a, the CD172b protein does not possess the cytoplasmic domain, but instead its transmembrane domain can interact with another transmembrane protein DAP-12, which contains ITAM sequences in its intracellular domain and links CD172b to the downstream signaling molecules. The result is e.g. regulation of neutrophil transepithelial migration.

Entrez Gene ID 10326

UniProt ID O00241

Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD172b (B4B6) PE.

Product specific references:

Baba N, Van VQ, Wakahara K, Rubio M, Fortin G, Panzini B, Soucy G, Wassef R, Richard C, Tamaz R, Lahaie R, Bernard EJ, Caussignac Y, Leduc R, Lougnarath R, Bergeron C, Racicot MA, Bergeron F, Panzini MA, Demetter P, Franchimont D, Schäkel K, Weckbecker G, Kolbinger F, Heusser C, Huber T, Welzenbach K, Sarfati M: CD47 fusion protein targets CD172a+ cells in Crohn's disease and dampens the production of IL-1β and TNF. J Exp Med. 2013 Jun 3;210(6):1251-63.
PubMed