Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD172ab Purified

Anti-Hu CD172ab Purified

Regulatory status

RUO

Antigen

CD172ab

Clone

SE5A5

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-133-C100

In stock

143.00 USD

0.025 mg

11-133-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-133-C100

In stock

143.00 USD

0.025 mg

11-133-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody SE5A5 recognizes a common extracellular epitope on human CD172a and CD172b antigens (approx. 90 kDa and approx. 50 kDa, respectively), although its reactivity with CD172a is higher.

Workshop

HLDA VII: WS Code 70259

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

NIH-3T3 / human CD172a cell line

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

PTPNS1, BIT, MFR, SIRPA, SHPS1, SIRPB1,

Antigen description

CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. Its extracellular ligand is CD47. CD172a serves as a substrate of activated receptor tyrosine kinases and upon phosphorylation it recruits SH2 domain-containing tyrosine phosphatases, thereby regulating signal transduction processes related to cell activation, transmigration and phagocytosis. CD172a is a specific marker of cardiomyocytes derived from human pluripotent stem cells and serves as a negative regulator of signaling and growth in myeloid progenitor cells. Extracellular part of CD172b is 90% identical to that of CD172a, but unlike CD172, it has dramatically reduced intracellular domain.

Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD172a/b (SE5A5) purified, GAM-APC.

Product specific references:

Suparak S, Muangsombut V, Riyapa D, Stevens JM, Stevens MP, Lertmemongkolchai G, Korbsrisate S: Burkholderia pseudomallei-induced cell fusion in U937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules. Microbes Infect. 2011 Nov;13(12-13):1006-11.
PubMed

Orazizadeh M, Salter DM: The expression of signal regulatory protein-alpha in normal and osteoarthritic human articular cartilage and its involvement in chondrocyte mechano-transduction response. Iran Biomed J. 2007 Apr;11(2):119-24.
PubMed

Schernthaner GH, Hauswirth AW, Baghestanian M, Agis H, Ghannadan M, Worda C, Krauth MT, Printz D, Fritsch G, Sperr WR, Valent P: Detection of differentiation- and activation-linked cell surface antigens on cultured mast cell progenitors. Allergy. 2005 Oct;60(10):1248-55.
PubMed

Kim EJ, Suk K, Lee WH: SHPS-1 and a synthetic peptide representing its ITIM inhibit the MyD88, but not TRIF, pathway of TLR signaling through activation of SHP and PI3K in THP-1 cells. Inflamm Res. 2013 Apr;62(4):377-86.
PubMed

Brooke G, Holbrook JD, Brown MH, Barclay AN: Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family. J Immunol. 2004 Aug 15;173(4):2562-70.
PubMed

Hatherley D, Harlos K, Dunlop DC, Stuart DI, Barclay AN: The structure of the macrophage signal regulatory protein alpha (SIRPalpha) inhibitory receptor reveals a binding face reminiscent of that used by T cell receptors. J Biol Chem. 2007 May 11;282(19):14567-75.
PubMed

Ha B, Lv Z, Bian Z, Zhang X, Mishra A, Liu Y: 'Clustering' SIRPα into the plasma membrane lipid microdomains is required for activated monocytes and macrophages to mediate effective cell surface interactions with CD47. PLoS One. 2013 Oct 15;8(10):e77615.
PubMed

Florian S, Ghannadan M, Mayerhofer M, Aichberger KJ, Hauswirth AW, Schernthaner GH, Printz D, Fritsch G, Böhm A, Sonneck K, Krauth MT, Müller MR, Sillaber C, Sperr WR, Bühring HJ, Valent P: Evaluation of normal and neoplastic human mast cells for expression of CD172a (SIRPalpha), CD47, and SHP-1. J Leukoc Biol. 2005 Jun;77(6):984-92. Epub 2005 Mar 22.
PubMed