Anti-Hu CD172ab PE

Anti-Hu CD172ab PE
Regulatory status
RUO
Antigen
CD172ab
Clone
SE5A5
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1P-133-T100
In stock

25 tests
1P-133-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody SE5A5 recognizes a common extracellular epitope on human CD172a and CD172b antigens (approx. 90 kDa and approx. 50 kDa, respectively), although its reactivity with CD172a is higher.
Workshop
HLDA VII: WS Code 70259
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
NIH-3T3 / human CD172a cell line
Other names
PTPNS1, BIT, MFR, SIRPA, SHPS1, SIRPB1,
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
1P-133 FC
Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD172a/b (SE5A5) PE.

Product specific references:

Suparak S, Muangsombut V, Riyapa D, Stevens JM, Stevens MP, Lertmemongkolchai G, Korbsrisate S: Burkholderia pseudomallei-induced cell fusion in U937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules. Microbes Infect. 2011 Nov;13(12-13):1006-11.
PubMed
Orazizadeh M, Salter DM: The expression of signal regulatory protein-alpha in normal and osteoarthritic human articular cartilage and its involvement in chondrocyte mechano-transduction response. Iran Biomed J. 2007 Apr;11(2):119-24.
PubMed
Schernthaner GH, Hauswirth AW, Baghestanian M, Agis H, Ghannadan M, Worda C, Krauth MT, Printz D, Fritsch G, Sperr WR, Valent P: Detection of differentiation- and activation-linked cell surface antigens on cultured mast cell progenitors. Allergy. 2005 Oct;60(10):1248-55.
PubMed
Kim EJ, Suk K, Lee WH: SHPS-1 and a synthetic peptide representing its ITIM inhibit the MyD88, but not TRIF, pathway of TLR signaling through activation of SHP and PI3K in THP-1 cells. Inflamm Res. 2013 Apr;62(4):377-86.
PubMed
Brooke G, Holbrook JD, Brown MH, Barclay AN: Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family. J Immunol. 2004 Aug 15;173(4):2562-70.
PubMed
Hatherley D, Harlos K, Dunlop DC, Stuart DI, Barclay AN: The structure of the macrophage signal regulatory protein alpha (SIRPalpha) inhibitory receptor reveals a binding face reminiscent of that used by T cell receptors. J Biol Chem. 2007 May 11;282(19):14567-75.
PubMed
Ha B, Lv Z, Bian Z, Zhang X, Mishra A, Liu Y: 'Clustering' SIRPα into the plasma membrane lipid microdomains is required for activated monocytes and macrophages to mediate effective cell surface interactions with CD47. PLoS One. 2013 Oct 15;8(10):e77615.
PubMed
Florian S, Ghannadan M, Mayerhofer M, Aichberger KJ, Hauswirth AW, Schernthaner GH, Printz D, Fritsch G, Böhm A, Sonneck K, Krauth MT, Müller MR, Sillaber C, Sperr WR, Bühring HJ, Valent P: Evaluation of normal and neoplastic human mast cells for expression of CD172a (SIRPalpha), CD47, and SHP-1. J Leukoc Biol. 2005 Jun;77(6):984-92. Epub 2005 Mar 22.
PubMed
Variant
100 tests
1P-133-T100
In stock

25 tests
1P-133-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock