Anti-Hu CD170 Purified

Anti-Hu CD170 Purified
Regulatory status
RUO
Antigen
CD170
Clone
1A5
Format
Purified
Reactivity
Non-human primates, Human
Variant
0.1 mg
11-156-C100
In stock
170.00 USD

0.025 mg
11-156-C025
Delivery 1 week
85.00 USD
Variant
0.1 mg
11-261-C100
In stock
170.00 USD

0.1 mg
11-261-C100
In stock
85.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 1A5 recognizes an extracellular epitope of CD170 (Siglec-5, sialic acid binding Ig-like lectin 5), a transmembrane glycoprotein expressed strongly by neutrophils, macrophages activated during infections, monocytes, and dendritic cells. As in case with other anti-CD170 antibodies, this antibody crossreacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Workshop
HLDA VII: WS Code 70443
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Immunohistochemistry: Recommended dilution: 5-10 μg/ml.
Reactivity
Non-human primates, Human
Immunogen
Fusion protein composed of human CD170 extracellular domain and Fc region of human IgG1
Concentration
1 mg/ml
Preparation
Purified from cell culture supernatant by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
OBBP2, OB-BP2, SIGLEC-5, SIGLEC5
Antigen description
CD170, also known as Siglec 5 (sialic acid binding Ig-like lectin 5) is a type 1 transmembrane glycoprotein containing two cytoplasmic immunoreceptor tyrosine inhibitory motifs (ITIMs). CD170 forms homodimers and functions as an inhibitory receptor able to downregulate cell activation. It binds to alpha2,3- and alpha2,6-linked sialic acid ligands, e.g. on glycophorin A (CD235a). Aberrant expression of CD170 by CD34+ progenitor cells can be observed in case of acute myeloid leukemias.
Entrez Gene ID 8778
UniProt ID O15389
11-156 FC
Flow cytometry (surface staining) of human peripheral blood cells using anti-CD170 (1A5) purified, GAM-APC.

Product specific references:

Avril T, Freeman SD, Attrill H, Clarke RG, Crocker PR: Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation. J Biol Chem. 2005 May 20;280(20):19843-51.
PubMed
Yokoi H, Myers A, Matsumoto K, Crocker PR, Saito H, Bochner BS: Alteration and acquisition of Siglecs during in vitro maturation of CD34+ progenitors into human mast cells. Allergy. 2006 Jun;61(6):769-76.
PubMed
Angata T, Hayakawa T, Yamanaka M, Varki A, Nakamura M: Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates. FASEB J. 2006 Oct;20(12):1964-73.
PubMed
Schuster P, Donhauser N, Pritschet K, Ries M, Haupt S, Kittan NA, Korn K, Schmidt B: Co-ordinated regulation of plasmacytoid dendritic cell surface receptors upon stimulation with herpes simplex virus type 1. Immunology. 2010 Feb;129(2):234-47.
PubMed
Variant
0.1 mg
11-156-C100
In stock
170.00 USD

0.025 mg
11-156-C025
Delivery 1 week
85.00 USD
Variant
0.1 mg
11-261-C100
In stock
170.00 USD

0.1 mg
11-261-C100
In stock
85.00 USD

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