Anti-Hu CD170 PE

Anti-Hu CD170 PE
Regulatory status
RUO
Antigen
CD170
Clone
1A5
Format
PE
Reactivity
Non-human primates, Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-156-T100
Ask for delivery term HERE
242.00 USD

25 tests
1P-156-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-156-T100
Ask for delivery term HERE
242.00 USD

25 tests
1P-156-T025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 1A5 recognizes an extracellular epitope of CD170 (Siglec-5, sialic acid binding Ig-like lectin 5), a transmembrane glycoprotein expressed strongly by neutrophils, macrophages activated during infections, monocytes, and dendritic cells. As in case with other anti-CD170 antibodies, this antibody crossreacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Workshop
HLDA VII: WS Code 70443
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Non-human primates, Human
Immunogen
Fusion protein composed of human CD170 extracellular domain and Fc region of human IgG1
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
OBBP2, OB-BP2, SIGLEC-5, SIGLEC5
Antigen description
CD170, also known as Siglec 5 (sialic acid binding Ig-like lectin 5) is a type 1 transmembrane glycoprotein containing two cytoplasmic immunoreceptor tyrosine inhibitory motifs (ITIMs). CD170 forms homodimers and functions as an inhibitory receptor able to downregulate cell activation. It binds to alpha2,3- and alpha2,6-linked sialic acid ligands, e.g. on glycophorin A (CD235a). Aberrant expression of CD170 by CD34+ progenitor cells can be observed in case of acute myeloid leukemias.
Entrez Gene ID 8778
UniProt ID O15389
1P-156 FC
Flow cytometry analysis (surface staining) of human peripheral blood cells using anti-CD170 (1A5) PE.

Product specific references:

Avril T, Freeman SD, Attrill H, Clarke RG, Crocker PR: Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation. J Biol Chem. 2005 May 20;280(20):19843-51.
PubMed
Yokoi H, Myers A, Matsumoto K, Crocker PR, Saito H, Bochner BS: Alteration and acquisition of Siglecs during in vitro maturation of CD34+ progenitors into human mast cells. Allergy. 2006 Jun;61(6):769-76.
PubMed
Angata T, Hayakawa T, Yamanaka M, Varki A, Nakamura M: Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates. FASEB J. 2006 Oct;20(12):1964-73.
PubMed
Schuster P, Donhauser N, Pritschet K, Ries M, Haupt S, Kittan NA, Korn K, Schmidt B: Co-ordinated regulation of plasmacytoid dendritic cell surface receptors upon stimulation with herpes simplex virus type 1. Immunology. 2010 Feb;129(2):234-47.
PubMed
Variant
100 tests
1P-156-T100
Ask for delivery term HERE
242.00 USD

25 tests
1P-156-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-156-T100
Ask for delivery term HERE
242.00 USD

25 tests
1P-156-T025
Delivery 1 week
121.00 USD

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