Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD17 Purified

Anti-Hu CD17 Purified

Regulatory status

RUO

Antigen

CD17

Clone

MEM-68

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-666-C100

In stock

143.00 USD

0.025 mg

11-666-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-666-C100

In stock

143.00 USD

0.025 mg

11-666-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The mouse monoclonal antibody MEM-68 recognizes CD17, an extracellular membrane lipid moiety lactosylceramide expressed on granulocytes, monocytes and platelets.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 2 μg/ml.

Reactivity

Human

Immunogen

Pre-B cell line NALM-6

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Lactosylceramide

Antigen description

CD17, lactosylceramide, is an ubiquitous glycosphingolipid with uncharged disaccharide headgroup, highly enriched in lipid raft-derived structures. Besides playing a pivotal role in the biosynthesis of complex glycosphingilipids, lactosylceramide is involved in cell-cell and cell-matrix interactions and in signaling events linked to cell differentiation, development, apoptosis and oncogenesis. Lactosylceramide regulates integrin functions and production of nitric oxide. Its expression defines successive stages in the maturation of myeloid cells.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD17 (MEM-68) purified antibody (concentration in sample 9 μg/ml, GAM APC).

Separation of human neutrophil granulocytes (red-filled) from CD17 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD17 (MEM-68) purified antibody (concentration in sample 9 μg/ml, GAM APC).

General references:

Sharma DK, Brown JC, Cheng Z, Holicky EL, Marks DL, Pagano RE: The glycosphingolipid, lactosylceramide, regulates beta1-integrin clustering and endocytosis. Cancer Res. 2005 Sep 15;65(18):8233-41.
PubMed

Pannu R, Singh AK, Singh I: A novel role of lactosylceramide in the regulation of tumor necrosis factor alpha-mediated proliferation of rat primary astrocytes. Implications for astrogliosis following neurotrauma. J Biol Chem. 2005 Apr 8;280(14):13742-51.

Zhai X, Li XM, Momsen MM, Brockman HL, Brown RE: Lactosylceramide: lateral interactions with cholesterol. Biophys J. 2006 Oct 1;91(7):2490-500.
PubMed

Spychalska J, Smoleńska-Sym G, Zdebska E, Woźniak J, Kościelak J: Quantitative analysis of LacCer/CDw17 in human myelogenous leukaemic cells. Cell Mol Biol Lett. 2003;8(4):911-7.
PubMed

Bhunia AK, Arai T, Bulkley G, Chatterjee S: Lactosylceramide mediates tumor necrosis factor-alpha-induced intercellular adhesion molecule-1 (ICAM-1) expression and the adhesion of neutrophil in human umbilical vein endothelial cells. J Biol Chem. 1998 Dec 18;273(51):34349-57.
PubMed