Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD169 Purified

Anti-Hu CD169 Purified

Regulatory status

RUO

Antigen

CD169

Clone

7-239

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested), IHC(F)

Variant

0.1 mg

11-797-C100

In stock

130.00 USD

0.025 mg

11-797-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-261-C100

In stock

130.00 USD

0.1 mg

11-261-C100

In stock

65.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 7-239 recognizes an extracellular epitope of CD169 (sialoadhesin, Siglec-1), a 210 kDa type I transmembrane glycoprotein expressed on macrophages and dendritic cells.

Application

IP, WB, FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Immunogen

human rhinovirus 14-infected monocyte-derived dendritic cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SIGLEC1, SN, sialoadhesin

Antigen description

CD169, also known as Siglec-1 or sialoadhesin, is a type I transmembrane glycoprotein of the sialic acid binding Ig-like lectin family. It binds to sialylated glycoproteins on various haematopoietic cells to mediate cell-cell interactions. CD169 is expressed on a subset of macrophages and dendritic cells. On CD14+ monocytes its expression can be induced by interferon alpha and gamma. High expression of CD169 is observed in the spleen, lymph nodes, bone marrow, and under inflammatory conditions rheumatoid arthritis and atherosclerosis, lower in the liver, lungs and gut. It has been shown to be involved in antigen presentation to invariant NKT cells, which play an important role in the innate arm of the immune system to modulate the subsequent acquired immune responses.

Entrez Gene ID 6614

UniProt ID Q9BZZ2

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD169 (7-239) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Separation of human monocytes (red-filled) from CD169 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD169 (7-239) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Product specific references:

Kawasaki N, Vela JL, Nycholat CM, Rademacher C, Khurana A, van Rooijen N, Crocker PR, Kronenberg M, Paulson JC: Targeted delivery of lipid antigen to macrophages via the CD169/sialoadhesin endocytic pathway induces robust invariant natural killer T cell activation. Proc Natl Acad Sci U S A. 2013 May 7;110(19):7826-31.
PubMed

Schrauf C, Kirchberger S, Majdic O, Seyerl M, Zlabinger GJ, Stuhlmeier KM, Sachet M, Seipelt J, Stöckl J: The ssRNA genome of human rhinovirus induces a type I IFN response but fails to induce maturation in human monocyte-derived dendritic cells. J Immunol. 2009 Oct 1;183(7):4440-8.
PubMed

Variant

0.1 mg

11-797-C100

In stock

130.00 USD

0.025 mg

11-797-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-261-C100