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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD169 Purified
Anti-Hu CD169 Purified
Regulatory status
RUO
Antigen
CD169
Clone
7-239
Format
Purified
Reactivity
Human
Application
IP, WB, FC (QC tested), IHC(F)
Variant
0.1 mg
11-797-C100
In stock
130.00 USD
0.025 mg
11-797-C025
Out of stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
0.1 mg
11-261-C100
In stock
65.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 7-239 recognizes an extracellular epitope of CD169 (sialoadhesin, Siglec-1), a 210 kDa type I transmembrane glycoprotein expressed on macrophages and dendritic cells.
Application
IP, WB, FC (QC tested), IHC(F)
Application details
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Human
Immunogen
human rhinovirus 14-infected monocyte-derived dendritic cells
Other names
SIGLEC1, SN, sialoadhesin
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD169 (7-239) purified antibody (concentration in sample 1 μg/ml, GAM APC).
Separation of human monocytes (red-filled) from CD169 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD169 (7-239) purified antibody (concentration in sample 1 μg/ml, GAM APC).
Product specific references:
Kawasaki N, Vela JL, Nycholat CM, Rademacher C, Khurana A, van Rooijen N, Crocker PR, Kronenberg M, Paulson JC: Targeted delivery of lipid antigen to macrophages via the CD169/sialoadhesin endocytic pathway induces robust invariant natural killer T cell activation. Proc Natl Acad Sci U S A. 2013 May 7;110(19):7826-31.
PubMed
Schrauf C, Kirchberger S, Majdic O, Seyerl M, Zlabinger GJ, Stuhlmeier KM, Sachet M, Seipelt J, Stöckl J: The ssRNA genome of human rhinovirus induces a type I IFN response but fails to induce maturation in human monocyte-derived dendritic cells. J Immunol. 2009 Oct 1;183(7):4440-8.
PubMed
Variant
0.1 mg
11-797-C100
In stock
130.00 USD
0.025 mg
11-797-C025
Out of stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
0.1 mg
11-261-C100
In stock
65.00 USD
Contact distributor
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