Anti-Hu CD169 APC

Anti-Hu CD169 APC
Regulatory status
RUO
Antigen
CD169
Clone
7-239
Format
APC
Reactivity
Human
Application
FC (QC tested)
Excitation laser
red (633 nm)
Variant
100 tests
1A-797-T100
In stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 7-239 recognizes an extracellular epitope of CD169 (sialoadhesin, Siglec-1), a 210 kDa type I transmembrane glycoprotein expressed on macrophages and dendritic cells.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
human rhinovirus 14-infected monocyte-derived dendritic cells
Other names
SIGLEC1, SN, sialoadhesin
Preparation
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1A-797_FC_Profil Značení
Flow cytometry surface staining pattern of human TNF-α and INF-γ stimulated peripheral blood mononuclear cells stained using anti-human CD169 (7-239) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
1A-797_FC_Dot-plot
Flow cytometry multicolor surface staining of human TNF-α and INF-γ stimulated peripheral blood mononuclear cells stained using anti-human CD169 (7-239) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD11c (BU15) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).
1A-797_FC_Histogram
Separation of human CD169 positive CD11c positive cells (red-filled) from CD169 negative CD11c negative cells (black-dashed) in flow cytometry analysis (surface staining) of human TNF-α and INF-γ stimulated peripheral blood mononuclear cells stained using anti-human CD169 (7-239) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Product specific references:

Kawasaki N, Vela JL, Nycholat CM, Rademacher C, Khurana A, van Rooijen N, Crocker PR, Kronenberg M, Paulson JC: Targeted delivery of lipid antigen to macrophages via the CD169/sialoadhesin endocytic pathway induces robust invariant natural killer T cell activation. Proc Natl Acad Sci U S A. 2013 May 7;110(19):7826-31.
PubMed
Schrauf C, Kirchberger S, Majdic O, Seyerl M, Zlabinger GJ, Stuhlmeier KM, Sachet M, Seipelt J, Stöckl J: The ssRNA genome of human rhinovirus induces a type I IFN response but fails to induce maturation in human monocyte-derived dendritic cells. J Immunol. 2009 Oct 1;183(7):4440-8.
PubMed
Variant
100 tests
1A-797-T100
In stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD