Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD167a PE

Anti-Hu CD167a PE

Regulatory status

RUO

Antigen

CD167a

Clone

51D6

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-132-T100

In stock

220.00 USD

25 tests

1P-132-T025

Delivery 1 week

110.00 USD

Variant

100 tests

1P-132-T100

In stock

220.00 USD

25 tests

1P-132-T025

Delivery 1 week

110.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The mouse monoclonal antibody 51D6 recognizes an extracellular epitope of CD167a, an approximately 97-101 kDa receptor tyrosine kinase expressed mainly on epithelial cells, but also on B cells and dendritic cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

CD167a-transfected NIH-3T3 cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

EDDR1, MCK10, NTRK4, PTK3A, RTK6, CAK, TRKE, DDR1

Antigen description

CD167a, also known as e.g. discoidin domain receptor tyrosine kinase 1 (DDR1), tyrosine kinase receptor E (TRKE), cell adhesion kinase (CAK), or neuroepithelial tyrosine kinase 4 (NEP, NTRK4), is a transmembrane receptor tyrosine kinase expressed predominantly in epithelial cells. It has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms of this protein have been described. After binding to fibrilar collagens I, II, III, V, or basement membrane collagens IV and VIII, CD167a becomes activated and autophosphorylated and transduces collagen-induced signaling.

Entrez Gene ID 780

UniProt ID Q08345

Flow cytometry analysis (surface staining) of MCF-7 cell line stained with anti-human CD167a (51D6) PE (red) in comparison with FMO (fluorescence minus one) sample (black-dashed).

Product specific references:

Masuda H, Anwar SS, Bühring HJ, Rao JR, Gargett CE: A novel marker of human endometrial mesenchymal stem-like cells. Cell Transplant. 2012;21(10):2201-14.
PubMed