Anti-Hu CD166 PE

Anti-Hu CD166 PE
Regulatory status
RUO
Antigen
CD166
Clone
3A6
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-855-T100
In stock
242.00 USD
Variant
100 tests
1P-855-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 3A6 recognizes an extracellular epitope of CD166, a type I transmembrane glycoprotein expressed on neurons, activated T cells, activated monocytes, endothelium, epithelium, endometrium, fibroblasts, pluripotent stem cells, and blastocysts.
Workshop
HLDA VI
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
cultured human thymic epithelial cells
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
MEMD, ALCAM, KG-CAM
Antigen description
CD166 is a type 1 transmembrane glycoprotein, which is anchored to the actin cytoskeleton through its intracellular domain, whereas its extracellular part binds its ligand CD6. CD166 functions as an adhesion molecule expressed during development of many organs, as well as in hematopoiesis (pluripotent stem cells), endothelial and epithelial linings, endometrium, blastocysts, and in the central and peripheral nervous system (on neurons), as well as on activated T cells and activated monocytes. Multiple isoforms of CD166 have been found.
Entrez Gene ID 214
UniProt ID Q13740
1P-855_FC_Histogram
Separation of HUVEC cells stained using anti-human CD166 (3A6) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from HUVEC cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 9 μg/ml, same as CD166 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of HUVEC cell suspension.

Product specific references:

Leukocyte Typing VI., Kishimoto T et al. (editors), Garland Publishing Inc. (1997).
Kijima N, Hosen N, Kagawa N, Hashimoto N, Nakano A, Fujimoto Y, Kinoshita M, Sugiyama H, Yoshimine T: CD166/activated leukocyte cell adhesion molecule is expressed on glioblastoma progenitor cells and involved in the regulation of tumor cell invasion. Neuro Oncol. 2012 Oct;14(10):1254-64.
PubMed
Piazza T, Cha E, Bongarzone I, Canevari S, Bolognesi A, Polito L, Bargellesi A, Sassi F, Ferrini S, Fabbi M: Internalization and recycling of ALCAM/CD166 detected by a fully human single-chain recombinant antibody. J Cell Sci. 2005 Apr 1;118(Pt 7):1515-25.
PubMed
Levesque MC, Heinly CS, Whichard LP, Patel DD: Cytokine-regulated expression of activated leukocyte cell adhesion molecule (CD166) on monocyte-lineage cells and in rheumatoid arthritis synovium. Arthritis Rheum. 1998 Dec;41(12):2221-9.
PubMed
Pretzel D, Linss S, Rochler S, Endres M, Kaps C, Alsalameh S, Kinne RW: Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage. Arthritis Res Ther. 2011 Apr 15;13(2):R64
PubMed
Virtanen S, Schulte R, Stingl J, Caldas C, Shehata M: High-throughput surface marker screen on primary human breast tissues reveals further cellular heterogeneity. Breast Cancer Res. 2021 Jun 13;23(1):66.
PubMed
Variant
100 tests
1P-855-T100
In stock
242.00 USD
Variant
100 tests
1P-855-T100
In stock
242.00 USD

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