Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD166 PE

Anti-Hu CD166 PE

Regulatory status

RUO

Antigen

CD166

Clone

3A6

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-855-T100

In stock

242.00 USD

Variant

100 tests

1P-855-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 3A6 recognizes an extracellular epitope of CD166, a type I transmembrane glycoprotein expressed on neurons, activated T cells, activated monocytes, endothelium, epithelium, endometrium, fibroblasts, pluripotent stem cells, and blastocysts.

Workshop

HLDA VI

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

cultured human thymic epithelial cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

MEMD, ALCAM, KG-CAM

Antigen description

CD166 is a type 1 transmembrane glycoprotein, which is anchored to the actin cytoskeleton through its intracellular domain, whereas its extracellular part binds its ligand CD6. CD166 functions as an adhesion molecule expressed during development of many organs, as well as in hematopoiesis (pluripotent stem cells), endothelial and epithelial linings, endometrium, blastocysts, and in the central and peripheral nervous system (on neurons), as well as on activated T cells and activated monocytes. Multiple isoforms of CD166 have been found.

Entrez Gene ID 214

UniProt ID Q13740

Separation of HUVEC cells stained using anti-human CD166 (3A6) PE antibody (concentration in sample 5 μg/ml, red-filled) from HUVEC cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as CD166 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of HUVEC cell suspension.

Product specific references:

Leukocyte Typing VI., Kishimoto T et al. (editors), Garland Publishing Inc. (1997).

Kijima N, Hosen N, Kagawa N, Hashimoto N, Nakano A, Fujimoto Y, Kinoshita M, Sugiyama H, Yoshimine T: CD166/activated leukocyte cell adhesion molecule is expressed on glioblastoma progenitor cells and involved in the regulation of tumor cell invasion. Neuro Oncol. 2012 Oct;14(10):1254-64.
PubMed

Piazza T, Cha E, Bongarzone I, Canevari S, Bolognesi A, Polito L, Bargellesi A, Sassi F, Ferrini S, Fabbi M: Internalization and recycling of ALCAM/CD166 detected by a fully human single-chain recombinant antibody. J Cell Sci. 2005 Apr 1;118(Pt 7):1515-25.
PubMed

Levesque MC, Heinly CS, Whichard LP, Patel DD: Cytokine-regulated expression of activated leukocyte cell adhesion molecule (CD166) on monocyte-lineage cells and in rheumatoid arthritis synovium. Arthritis Rheum. 1998 Dec;41(12):2221-9.
PubMed

Pretzel D, Linss S, Rochler S, Endres M, Kaps C, Alsalameh S, Kinne RW: Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage. Arthritis Res Ther. 2011 Apr 15;13(2):R64
PubMed

Virtanen S, Schulte R, Stingl J, Caldas C, Shehata M: High-throughput surface marker screen on primary human breast tissues reveals further cellular heterogeneity. Breast Cancer Res. 2021 Jun 13;23(1):66.
PubMed