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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD165 Purified
Anti-Hu CD165 Purified
Regulatory status
RUO
Antigen
CD165
Clone
SN2
Format
Purified
Reactivity
Human
Application
IP, WB, FC (QC tested), IHC(F), ICC
Variant
0.1 mg
11-165-C100
In stock
150.00 USD
0.025 mg
11-165-C025
Out of stock
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD
0.1 mg
11-261-C100
In stock
75.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody SN2, also known as SN2 N6-D11, recognizes an extracellular epitope of CD165, an approximately 37-42 kDa transmembrane glycoprotein expressed mainly on leukemic T cells, double positive and double negative thymocytes (CD4-CD8-, CD4+CD8+), and platelets.
Workshop
HLDA VI: WS Code N-L099
Application
IP, WB, FC (QC tested), IHC(F), ICC
Application details
Immunohistochemistry (frozen sections): Application note: acetone fixation.
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Human
Immunogen
CD165 purified from human Molt-4 cell line
Other names
AD2
Concentration
1 mg/ml
Preparation
Purified from cell culture supernatant by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Separation of Jurkat cells stained using anti-human CD165 (SN2) purified antibody (concentration in sample 0,6 μg/ml, GAM APC, red) from Jurkat cells unstained by primary antibody (GAM APC, black) in flow cytometry analysis (surface staining).
Product specific references:
Seon BK: Specific killing of human T-leukemia cells by immunotoxins prepared with ricin A chain and monoclonal anti-human T-cell leukemia antibodies. Cancer Res. 1984 Jan;44(1):259-64.
PubMed
Seon BK, Negoro S, Barcos MP, Tebbi CK, Chervinsky D, Fukukawa T: Monoclonal antibody SN2 defining a human T cell leukemia-associated cell surface glycoprotein. J Immunol. 1984 Apr;132(4):2089-95.
PubMed
Variant
0.1 mg
11-165-C100
In stock
150.00 USD
0.025 mg
11-165-C025
Out of stock
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD
0.1 mg
11-261-C100
In stock
75.00 USD
Contact distributor
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