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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD165 Purified
Anti-Hu CD165 Purified
Regulatory status
RUO
Antigen
CD165
Clone
SN2
Format
Purified
Reactivity
Human
Application
IP, WB, FC (QC tested), IHC-F, ICC
Variant
0.1 mg
11-165-C100
In stock
169.40 USD
Variant
0.1 mg
11-165-C100
In stock
169.40 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody SN2, also known as SN2 N6-D11, recognizes an extracellular epitope of CD165, an approximately 37-42 kDa transmembrane glycoprotein expressed mainly on leukemic T cells, double positive and double negative thymocytes (CD4-CD8-, CD4+CD8+), and platelets.
Workshop
HLDA VI: WS Code N-L099
Application
IP, WB, FC (QC tested), IHC-F, ICC
Application details
Immunohistochemistry (frozen sections): Application note: acetone fixation.
Flow cytometry: Recommended dilution: 1-4 µg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.
Reactivity
Human
Immunogen
CD165 purified from human Molt-4 cell line
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
AD2
Antigen description
CD165 is a poorly characterized transmembrane protein highly expressed on platelets and many leukemic T cell lines. At lower level it is expressed on a proportion of circulating T cells and monocytes, on thymic epithelium, fibroblasts, epidermal keratinocytes, pancreatic islet cells, and some neurons. It might have a role in adhesion between thymocytes and thymic epithelial cells and it can be used as a marker for tumor progression.
Entrez Gene ID
23449
Separation of Jurkat cells stained using anti-human CD165 (SN2) purified antibody (concentration in sample 0,6 μg/ml, GAM APC, red) from Jurkat cells unstained by primary antibody (GAM APC, black) in flow cytometry analysis (surface staining).
Anti-Hu CD165 Purified (clone SN2) works in WB application under non-reducing conditions.
Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Molt-4, Raji, and Hep-G2 cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.
Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody SN2 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.
CD165 molecules were detected at ~34-48 kDa in Jurkat and Molt-4 cell lines under non-reducing conditions.
Product specific references:
Seon BK: Specific killing of human T-leukemia cells by immunotoxins prepared with ricin A chain and monoclonal anti-human T-cell leukemia antibodies. Cancer Res. 1984 Jan;44(1):259-64.
PubMed
Seon BK, Negoro S, Barcos MP, Tebbi CK, Chervinsky D, Fukukawa T: Monoclonal antibody SN2 defining a human T cell leukemia-associated cell surface glycoprotein. J Immunol. 1984 Apr;132(4):2089-95.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
11-165-C100
In stock
169.40 USD
Variant
0.1 mg
11-165-C100
In stock
169.40 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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