Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD165 Purified

Anti-Hu CD165 Purified

Regulatory status

RUO

Antigen

CD165

Clone

SN2

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested), IHC(F), ICC

Variant

0.1 mg

11-165-C100

Ask for delivery term HERE

165.00 USD

Variant

0.1 mg

11-165-C100

Ask for delivery term HERE

165.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody SN2, also known as SN2 N6-D11, recognizes an extracellular epitope of CD165, an approximately 37-42 kDa transmembrane glycoprotein expressed mainly on leukemic T cells, double positive and double negative thymocytes (CD4-CD8-, CD4+CD8+), and platelets.

Workshop

HLDA VI: WS Code N-L099

Application

IP, WB, FC (QC tested), IHC(F), ICC

Application details

Immunohistochemistry (frozen sections): Application note: acetone fixation.
Flow cytometry: Recommended dilution: 1-4 µg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.

Reactivity

Human

Immunogen

CD165 purified from human Molt-4 cell line

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

AD2

Antigen description

CD165 is a poorly characterized transmembrane protein highly expressed on platelets and many leukemic T cell lines. At lower level it is expressed on a proportion of circulating T cells and monocytes, on thymic epithelium, fibroblasts, epidermal keratinocytes, pancreatic islet cells, and some neurons. It might have a role in adhesion between thymocytes and thymic epithelial cells and it can be used as a marker for tumor progression.

Entrez Gene ID 23449

Separation of Jurkat cells stained using anti-human CD165 (SN2) purified antibody (concentration in sample 0,6 μg/ml, GAM APC, red) from Jurkat cells unstained by primary antibody (GAM APC, black) in flow cytometry analysis (surface staining).

Anti-Hu CD165 Purified (clone SN2) works in WB application under non-reducing conditions.

Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Molt-4, Raji, and Hep-G2 cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody SN2 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.

CD165 molecules were detected at ~34-48 kDa in Jurkat and Molt-4 cell lines under non-reducing conditions.

Product specific references:

Seon BK: Specific killing of human T-leukemia cells by immunotoxins prepared with ricin A chain and monoclonal anti-human T-cell leukemia antibodies. Cancer Res. 1984 Jan;44(1):259-64.
PubMed

Seon BK, Negoro S, Barcos MP, Tebbi CK, Chervinsky D, Fukukawa T: Monoclonal antibody SN2 defining a human T cell leukemia-associated cell surface glycoprotein. J Immunol. 1984 Apr;132(4):2089-95.
PubMed