Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD164 Purified

Anti-Hu CD164 Purified

Regulatory status

RUO

Antigen

CD164

Clone

67D2

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested), ICC

Variant

0.1 mg

11-135-C100

In stock

143.00 USD

0.025 mg

11-135-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-135-C100

In stock

143.00 USD

0.025 mg

11-135-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 67D2 recognizes an extracellular class III epitope (not sensitive to sialidase, N-glycanase, O-glycosidase, and O-sialoglycoprotease) of CD164, a sialomucin expressed in hematopoietic myeloid and erythroid progenitors, activated basophils, and in various carcinomas and leukemic cells.

Workshop

HLDA VIII

Application

IP, WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.
Western blotting: Recommended dilution: 1-2 µg/ml.

Reactivity

Human

Immunogen

Breast tumor cell line T-47D

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

DFNA66, MUC-24, MGC-24, endolyn

Antigen description

CD164, also known as endolyn, is a type I transmembrane protein with heavily glycosylated extracellular part containing sialic acid and glycosaminoglycan residues. CD164 plays both adhesive and antiadhesive role and serves as a potent negative regulator for CD34+ CD38- hematopoietic progenitor cell proliferation. It has also been reported to be involved in myogenic differentiation and cancer metastasis. The adhesive and negative regulatory functions seem to depend on different posttranslational modifications of CD164 protein.

Entrez Gene ID 8763

UniProt ID Q04900

Flow cytometry analysis (surface staining) of human peripheral blood cells using anti-CD164 (67D2) purified, GAM-FITC.

Western blotting analysis of human CD164 expression in various cell lines under reducing and non-reducing conditions using mouse monoclonal antibody 67D2. The same amount of total protein (20 μg) was loaded in each lane. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody.

Product specific references:

Doyonnas R, Yi-Hsin Chan J, Butler LH, Rappold I, Lee-Prudhoe JE, Zannettino AC, Simmons PJ, Bühring HJ, Levesque JP, Watt SM: CD164 monoclonal antibodies that block hemopoietic progenitor cell adhesion and proliferation interact with the first mucin domain of the CD164 receptor. J Immunol. 2000 Jul 15;165(2):840-51.
PubMed

Jorgensen-Tye B, Levesque JP, Royle L, Doyonnas R, Chan JY, Dwek RA, Rudd PM, Harvey DJ, Simmons PJ, Watt SM: Epitope recognition of antibodies that define the sialomucin, endolyn (CD164), a negative regulator of haematopoiesis. Tissue Antigens. 2005 Mar;65(3):220-39.
PubMed

Pilling D, Vakil V, Gomer RH: Improved serum-free culture conditions for the differentiation of human and murine fibrocytes. J Immunol Methods. 2009 Dec 31;351(1-2):62-70.
PubMed

Hennersdorf F, Florian S, Jakob A, Baumgärtner K, Sonneck K, Nordheim A, Biedermann T, Valent P, Bühring HJ: Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation. Cell Res. 2005 May;15(5):325-35.
PubMed