Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD164 Purified

Anti-Hu CD164 Purified

Anti-Hu CD164 Purified
Regulatory status
RUO
Antigen
CD164
Clone
67D2
Format
Purified
Reactivity
Human
Application
IP, WB, FC (QC tested), ICC
Variant
0.1 mg
11-135-C100
In stock
130.00 USD
0.025 mg
11-135-C025
Out of stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
0.1 mg
11-261-C100
In stock
65.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 67D2 recognizes an extracellular class III epitope (not sensitive to sialidase, N-glycanase, O-glycosidase, and O-sialoglycoprotease) of CD164, a sialomucin expressed in hematopoietic myeloid and erythroid progenitors, activated basophils, and in various carcinomas and leukemic cells.
Workshop
HLDA VIII
Application
IP, WB, FC (QC tested), ICC
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Human
Immunogen
Breast tumor cell line T-47D
Other names
DFNA66, MUC-24, MGC-24, endolyn
Concentration
1 mg/ml
Preparation
Purified from cell culture supernatant by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-135_FC_Profil značení
Flow cytometry analysis (surface staining) of human peripheral blood cells using anti-CD164 (67D2) purified, GAM-FITC.

Product specific references:

Doyonnas R, Yi-Hsin Chan J, Butler LH, Rappold I, Lee-Prudhoe JE, Zannettino AC, Simmons PJ, Bühring HJ, Levesque JP, Watt SM: CD164 monoclonal antibodies that block hemopoietic progenitor cell adhesion and proliferation interact with the first mucin domain of the CD164 receptor. J Immunol. 2000 Jul 15;165(2):840-51.
PubMed
Jorgensen-Tye B, Levesque JP, Royle L, Doyonnas R, Chan JY, Dwek RA, Rudd PM, Harvey DJ, Simmons PJ, Watt SM: Epitope recognition of antibodies that define the sialomucin, endolyn (CD164), a negative regulator of haematopoiesis. Tissue Antigens. 2005 Mar;65(3):220-39.
PubMed
Pilling D, Vakil V, Gomer RH: Improved serum-free culture conditions for the differentiation of human and murine fibrocytes. J Immunol Methods. 2009 Dec 31;351(1-2):62-70.
PubMed
Hennersdorf F, Florian S, Jakob A, Baumgärtner K, Sonneck K, Nordheim A, Biedermann T, Valent P, Bühring HJ: Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation. Cell Res. 2005 May;15(5):325-35.
PubMed
Variant
0.1 mg
11-135-C100
In stock
130.00 USD
0.025 mg
11-135-C025
Out of stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
0.1 mg
11-261-C100
In stock
65.00 USD
Contact distributor
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Bulk requests
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