Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD162 Purified

Anti-Hu CD162 Purified

Regulatory status

RUO

Antigen

CD162

Clone

TC2

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-603-C100

In stock

154.00 USD

0.025 mg

11-603-C025

Delivery 1 week

77.00 USD

Variant

0.1 mg

11-603-C100

In stock

154.00 USD

0.025 mg

11-603-C025

Delivery 1 week

77.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody TC2 reacts with an extracellular epitope of CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes; it is also expressed on a subpopulation of B lymphocytes and CD34⁺ bone marrow cells.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1.5 - 2.5 μg/ml; positive control: human peripheral blood.

Reactivity

Human

Immunogen

Human thymocytes

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

PSGL-1, Selectin P ligand, SELPLG, CLA

Antigen description

CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors.

Entrez Gene ID 6404

UniProt ID Q14242

Flow cytometry (surface staining) of human peripheral blood cells with anti-human CD162 (clone TC2).

General references:

Moore KL: Structure and function of P-selectin glycoprotein ligand-1. Leuk Lymphoma. 1998 Mar;29(1-2):1-15.
PubMed

Lévesque JP, Zannettino AC, Pudney M, Niutta S, Haylock DN, Snapp KR, Kansas GS, Berndt MC, Simmons PJ. PSGL-1-mediated adhesion of human hematopoietic progenitors to P-selectin results in suppression of hematopoiesis. Immunity. 1999 Sep;11(3):369-78.
PubMed

Davenpeck KL, Brummet ME, Hudson SA, Mayer RJ, Bochner BS: Activation of human leukocytes reduces surface P-selectin glycoprotein ligand-1 (PSGL-1, CD162) and adhesion to P-selectin in vitro. J Immunol. 2000 Sep 1;165(5):2764-72.
PubMed

Marsik C, Mayr F, Cardona F, Schaller G, Wagner OF, Jilma B: Endotoxin down-modulates P-selectin glycoprotein ligand-1 (PSGL-1, CD162) on neutrophils in humans. J Clin Immunol. 2004 Jan;24(1):62-5.
PubMed

Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002).

Product specific references:

Wake H, Mori S, Liu K, Morioka Y, Teshigawara K, Sakaguchi M, Kuroda K, Gao Y, Takahashi H, Ohtsuka A, Yoshino T, Morimatsu H, Nishibori M: Histidine-Rich Glycoprotein Prevents Septic Lethality through Regulation of Immunothrombosis and Inflammation. EBioMedicine. 2016 Jul;9:180-194.
PubMed

Nakata K, Takeda S, Tanaka A, Kwang J, Komano J: Antiviral activity of acid beta-glucosidase 1 on enterovirus 71, a causative agent of hand, foot and mouth disease. J Gen Virol. 2017 Apr;98(4):643-651.
PubMed