Anti-Hu CD160 PE

Anti-Hu CD160 PE
Regulatory status
RUO
Antigen
CD160
Clone
BY55
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-750-T100
Ask for delivery term HERE
242.00 USD

25 tests
1P-750-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-750-T100
Ask for delivery term HERE
242.00 USD

25 tests
1P-750-T025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgM kappa
Specificity
The mouse monoclonal antibody BY55 recognizes an extracellular epitope of CD160, a 27 kDa glycoprotein expressed on NK cells, NK-T cells, intestinal intraepithelial lymphocytes, TCR-gamma/delta T cells and a small population of TCR-alpha/beta T cells. The antibody detects both GPI-anchored and transmembrane form of CD160.
Workshop
HCDM listed
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human NK cell line YT2C2
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
NK1, NK28
Antigen description
CD160 is a cell surface glycoprotein of immunoglobulin superfamily, which functions as a costimulatory receptor expressed mainly on cytotoxic cell populations and recognizing both classical and non-classical MHC class I molecules. It can form disulfide-linked multimers. Down-modulation of CD160 occurs as a consequence of its proteolytic cleavage and the released soluble form was found to impair the MHC-class I specific cytotoxicity of CD8+ T lymphocytes and NK cells. In contrast to GPI-anchored isoform with broader expression among CD160 positive cells, expression of the transmembrane isoform is restricted to NK cells and is activation-dependent.
Entrez Gene ID 11126
UniProt ID O95971
1P-750_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD160 (BY55) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-750_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD160 (BY55) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-750_FC_Histogram
Separation of human CD160 positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD160 (BY55) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Merino J, Ramírez N, Moreno C, Toledo E, Fernández M, Sánchez-Ibarrola A: BY55/CD160 cannot be considered a cytotoxic marker in cytomegalovirus-specific human CD8(+) T cells. Clin Exp Immunol. 2007 Jul;149(1):87-96.
PubMed
Anumanthan A, Bensussan A, Boumsell L, Christ AD, Blumberg RS, Voss SD, Patel AT, Robertson MJ, Nadler LM, Freeman GJ: Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes. J Immunol. 1998 Sep 15;161(6):2780-90.
PubMed
Maïza H, Leca G, Mansur IG, Schiavon V, Boumsell L, Bensussan A: A novel 80-kD cell surface structure identifies human circulating lymphocytes with natural killer activity. J Exp Med. 1993 Sep 1;178(3):1121-6.
PubMed
Giustiniani J, Bensussan A, Marie-Cardine A: Identification and characterization of a transmembrane isoform of CD160 (CD160-TM), a unique activating receptor selectively expressed upon human NK cell activation. J Immunol. 2009 Jan 1;182(1):63-71.
PubMed
Bensussan A, Gluckman E, el Marsafy S, Schiavon V, Mansur IG, Dausset J, Boumsell L, Carosella E: BY55 monoclonal antibody delineates within human cord blood and bone marrow lymphocytes distinct cell subsets mediating cytotoxic activity. Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):9136-40.
PubMed
Variant
100 tests
1P-750-T100
Ask for delivery term HERE
242.00 USD

25 tests
1P-750-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-750-T100
Ask for delivery term HERE
242.00 USD

25 tests
1P-750-T025
Delivery 1 week
121.00 USD

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