Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD160 Alexa Fluor<sup>®</sup> 647

Anti-Hu CD160 Alexa Fluor^® 647

Regulatory status

RUO

Antigen

CD160

Clone

BY55

Format

Alexa Fluor^® 647

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

A6-750-T100

In stock

242.00 USD

Variant

100 tests

A6-750-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM kappa

Specificity

The mouse monoclonal antibody BY55 recognizes an extracellular epitope of CD160, a 27 kDa glycoprotein expressed on NK cells, NK-T cells, intestinal intraepithelial lymphocytes, TCR-gamma/delta T cells and a small population of TCR-alpha/beta T cells. The antibody detects both GPI-anchored and transmembrane form of CD160.

Workshop

HCDM listed

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Human NK cell line YT2C2

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

NK1, NK28

Antigen description

CD160 is a cell surface glycoprotein of immunoglobulin superfamily, which functions as a costimulatory receptor expressed mainly on cytotoxic cell populations and recognizing both classical and non-classical MHC class I molecules. It can form disulfide-linked multimers. Down-modulation of CD160 occurs as a consequence of its proteolytic cleavage and the released soluble form was found to impair the MHC-class I specific cytotoxicity of CD8+ T lymphocytes and NK cells. In contrast to GPI-anchored isoform with broader expression among CD160 positive cells, expression of the transmembrane isoform is restricted to NK cells and is activation-dependent.

Entrez Gene ID 11126

UniProt ID O95971

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD160 (BY55) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD160 (BY55) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD56 (LT56) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD160 positive CD56 positive lymphocytes (red-filled) from human CD160 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD160 (BY55) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Merino J, Ramírez N, Moreno C, Toledo E, Fernández M, Sánchez-Ibarrola A: BY55/CD160 cannot be considered a cytotoxic marker in cytomegalovirus-specific human CD8(+) T cells. Clin Exp Immunol. 2007 Jul;149(1):87-96.
PubMed

Anumanthan A, Bensussan A, Boumsell L, Christ AD, Blumberg RS, Voss SD, Patel AT, Robertson MJ, Nadler LM, Freeman GJ: Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes. J Immunol. 1998 Sep 15;161(6):2780-90.
PubMed

Maïza H, Leca G, Mansur IG, Schiavon V, Boumsell L, Bensussan A: A novel 80-kD cell surface structure identifies human circulating lymphocytes with natural killer activity. J Exp Med. 1993 Sep 1;178(3):1121-6.
PubMed

Giustiniani J, Bensussan A, Marie-Cardine A: Identification and characterization of a transmembrane isoform of CD160 (CD160-TM), a unique activating receptor selectively expressed upon human NK cell activation. J Immunol. 2009 Jan 1;182(1):63-71.
PubMed

Bensussan A, Gluckman E, el Marsafy S, Schiavon V, Mansur IG, Dausset J, Boumsell L, Carosella E: BY55 monoclonal antibody delineates within human cord blood and bone marrow lymphocytes distinct cell subsets mediating cytotoxic activity. Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):9136-40.
PubMed