Anti-Hu CD16 Purified

Anti-human CD16 purified antibody (clone MEM-168) works in flow cytometry application.

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Mouse monoclonal anti-human CD16 purified antibody (clone MEM-168) was used in concentration 2 µg/ml in stained blood sample (2 x 10⁶ cells).

Expression profiling on peripheral blood subsets using anti-human CD16 purified antibody (clone MEM-168).

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 10⁶ cells) with residual erythrocytes lysed with 10× diluted EXCELLYSE Live solution (#ED7068) was added to the mixture of anti-human CD16 purified antibody (clone MEM-168, 2 µg/ml in stained blood sample) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Next, samples were centrifuged (670 g, 5 min.), supernatant removed and secondary antibody (GAM PE) was added to sample, vortexed and incubated for 20 min. Next, samples were washed twice (2 ml PBS, 670 g, 5 min.) and then optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) were added to test tubes, vortexed and incubated for 20 min. Next, samples are fixed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Flow cytometry (surface staining) of lysed and washed porcine peripheral blood with purified anti-CD16 (MEM-168) (detection by anti-mouse IgM FITC). Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03; cat. no. 11-803-C100) Panel B, C, D - three different porcine PMBC samples stained with anti-CD16 (MEM-168) Cells in the granulocyte gate were used for analysis.