Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD16 Purified

Anti-Hu CD16 Purified

Regulatory status

RUO

Antigen

CD16

Clone

MEM-168

Format

Purified

Reactivity

Non-human primates, Human, Pig

Application

FC (QC tested)

Variant

0.1 mg

11-382-C100

In stock

130.00 USD

0.025 mg

11-382-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-261-C100

In stock

130.00 USD

0.1 mg

11-261-C100

In stock

65.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgM

Specificity

The antibody MEM-168 reacts with an extracellular epitope of CD16 antigen, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils).

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Non-human primates, Human, Pig

Immunogen

Human granulocytes

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

FcgammaRIII, IGFR3, FCRIII

Antigen description

CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

Flow cytometry (surface staining) of lysed and washed porcine peripheral blood with purified anti-CD16 (MEM-168) (detection by anti-mouse IgM FITC). Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03; cat. no. 11-803-C100) Panel B, C, D - three different porcine PMBC samples stained with anti-CD16 (MEM-168) Cells in the granulocyte gate were used for analysis.

General references:

Gessner JE, Grussenmeyer T, Kolanus W, Schmidt RE: The human low affinity immunoglobulin G Fc receptor III-A and III-B genes. Molecular characterization of the promoter regions. J Biol Chem. 1995 Jan 20;270(3):1350-61.
PubMed

Kocher M, Siegel ME, Edberg JC, Kimberly RP: Cross-linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J Immunol. 1997 Oct 15;159(8):3940-8.
PubMed

Arase N, Arase H, Hirano S, Yokosuka T, Sakurai D, Saito T: IgE-mediated activation of NK cells through Fc gamma RIII. J Immunol. 2003 Mar 15;170(6):3054-8.
PubMed

Product specific references:

Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84.
PubMed

Variant

0.1 mg

11-382-C100

In stock

130.00 USD

0.025 mg

11-382-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-261-C100