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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD16 Purified
Anti-Hu CD16 Purified
Regulatory status
RUO
Antigen
CD16
Clone
LNK16
Format
Purified
Reactivity
Non-human primates, Human
Application
IP, FC (QC tested)
Variant
0.1 mg
11-399-C100
In stock
143.00 USD
0.025 mg
11-399-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-399-C100
In stock
143.00 USD
0.025 mg
11-399-C025
Delivery 1 week
71.50 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody LNK16 reacts with an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils).
Workshop
HLDA V: WS Code M MA069; HLDA V: WS Code NK NK50
Application
IP, FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 3-12 μg/ml.
Reactivity
Non-human primates, Human
Immunogen
Normal human peripheral blood granulocytes
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
FcgammaRIII, IGFR3, FCRIII
Antigen description
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Anti-human CD16 purified antibody (clone LNK16) works in flow cytometry application.
Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Mouse monoclonal anti-human CD16 purified antibody (clone LNK16) was used in concentration 2 µg/ml in stained blood sample (2 x 10
6
cells).
Expression profiling on peripheral blood subsets using anti-human CD16 purified antibody (clone LNK-16).
HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 10
6
cells) with residual erythrocytes lysed with 10× diluted EXCELLYSE Live solution (#ED7068) was added to the mixture of anti-human CD16 purified antibody (clone LNK16, 2 µg/ml in stained blood sample) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Next, samples were centrifuged (670 g, 5 min.), supernatant removed and secondary antibody (GAM PE) was added to sample, vortexed and incubated for 20 min. Next, samples were washed twice (2 ml PBS, 670 g, 5 min.) and then optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) were added to test tubes, vortexed and incubated for 20 min. Next, samples are fixed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.
Flow cytometry (surface staining) of human peripheral blood cells with anti-CD16 (LNK16) purified / GAM-APC.
General references:
Gessner JE, Grussenmeyer T, Kolanus W, Schmidt RE: The human low affinity immunoglobulin G Fc receptor III-A and III-B genes. Molecular characterization of the promoter regions. J Biol Chem. 1995 Jan 20;270(3):1350-61.
PubMed
Kocher M, Siegel ME, Edberg JC, Kimberly RP: Cross-linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J Immunol. 1997 Oct 15;159(8):3940-8.
PubMed
Arase N, Arase H, Hirano S, Yokosuka T, Sakurai D, Saito T: IgE-mediated activation of NK cells through Fc gamma RIII. J Immunol. 2003 Mar 15;170(6):3054-8.
PubMed
Product specific references:
Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Tamm A, Schmidt RE: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 1996 Aug 15;157(4):1576-81.
PubMed
Boyle JJ: Human macrophages kill human mesangial cells by Fas-L-induced apoptosis when triggered by antibody via CD16. Clin Exp Immunol. 2004 Sep;137(3):529-37.
PubMed
Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106(10):3567-74.
PubMed
Mathison RD, Befus AD, Davison JS, Woodman RC: Modulation of neutrophil function by the tripeptide feG. BMC Immunol. 2003 Mar 4;4:3. Epub 2003 Mar 4.
PubMed
Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 2011 Jan 5;12:2.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
11-399-C100
In stock
143.00 USD
0.025 mg
11-399-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-399-C100
In stock
143.00 USD
0.025 mg
11-399-C025
Delivery 1 week
71.50 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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