Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD16 FITC

Anti-Hu CD16 FITC

Regulatory status

RUO

Antigen

CD16

Clone

LNK16

Format

FITC

Reactivity

Non-human primates, Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-399-T100

In stock

198.00 USD

25 tests

1F-399-T025

Delivery 1 week

99.00 USD

Variant

100 tests

1F-399-T100

In stock

198.00 USD

25 tests

1F-399-T025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody LNK16 reacts with an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils).

Workshop

HLDA V: WS Code M MA069; HLDA V: WS Code NK NK50

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reactivity

Non-human primates, Human

Immunogen

Normal human peripheral blood granulocytes

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

FcgammaRIII, IGFR3, FCRIII

Antigen description

CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

Anti-human CD16 FITC antibody (clone LNK16) works in flow cytometry application.

Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live (ED7068).
Mouse monoclonal anti-human CD16 FITC antibody (clone LNK16) was used in concentration 12 µg/ml in stained blood sample (2 x 10⁶ cells).

Staining pattern of Anti-human CD16 FITC antibody (clone LNK16) in dot-plot fluorescence visualization (lymphocyte gate)

Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live (ED7068).
Mouse monoclonal anti-human CD16 FITC antibody (clone LNK16) was used in concentration 12 µg/ml in stained stained blood sample (2 x 10⁶ cells) and Mouse monoclonal anti-human CD3 APC antibody (clone UCHT1) in concentration 6 µg/ml, respectively.

Staining pattern of Anti-human CD16 FITC antibody (clone LNK16) in dot-plot fluorescence visualization (mononuclear gate)

Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live (ED7068).
Mouse monoclonal anti-human CD16 FITC antibody (clone LNK16) was used in concentration 12 µg/ml in stained stained blood sample (2 x 10⁶ cells) and Mouse monoclonal anti-human CD14 PE-Cy™7 antibody (clone MEM-15) in concentration 8 µg/ml, respectively.

Reactivity of Anti-human CD16 FITC antibody (clone LNK16) on human peripheral leukocytes

Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live (ED7068).
Suspension of blood leukocytes (2 x 10⁶ cells) was added to the mixture of CD16 FITC antibody (clone LNK16, 12 µg/ml in stained blood sample), backbone antibody conjugates and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Stained sample was fixed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

General references:

Gessner JE, Grussenmeyer T, Kolanus W, Schmidt RE: The human low affinity immunoglobulin G Fc receptor III-A and III-B genes. Molecular characterization of the promoter regions. J Biol Chem. 1995 Jan 20;270(3):1350-61.
PubMed

Kocher M, Siegel ME, Edberg JC, Kimberly RP: Cross-linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J Immunol. 1997 Oct 15;159(8):3940-8.
PubMed

Arase N, Arase H, Hirano S, Yokosuka T, Sakurai D, Saito T: IgE-mediated activation of NK cells through Fc gamma RIII. J Immunol. 2003 Mar 15;170(6):3054-8.
PubMed

Product specific references:

Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

Tamm A, Schmidt RE: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 1996 Aug 15;157(4):1576-81.
PubMed

Boyle JJ: Human macrophages kill human mesangial cells by Fas-L-induced apoptosis when triggered by antibody via CD16. Clin Exp Immunol. 2004 Sep;137(3):529-37.
PubMed

Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106(10):3567-74.
PubMed

Mathison RD, Befus AD, Davison JS, Woodman RC: Modulation of neutrophil function by the tripeptide feG. BMC Immunol. 2003 Mar 4;4:3. Epub 2003 Mar 4.
PubMed

Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 2011 Jan 5;12:2.
PubMed