Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD158z PE

Anti-Hu CD158z PE

Regulatory status

RUO

Antigen

CD158z

Clone

CH21

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-945-C100

In stock

242.00 USD

Variant

0.1 mg

1P-945-C100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody CH21 recognizes an extracellular epitope of human CD158z (KIR3DL3), a transmembrane glycoprotein of killer cell inhibitory receptor family.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 µg/ml

Reactivity

Human

Immunogen

human CD158z transfectants

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

KIR3DL3

Antigen description

CD158z (KIR3DL3)is one of killer cell inhibitory receptors. It has three extracellular immunoglobulin-like domains and a long cytoplasmic tail, which, however, contains only one ITIM. Like other KIRs, CD158z is highly polymorphic, but it seems that its immunoglobulin-like domains are quite conserved among high primates.

Entrez Gene ID 115653

UniProt ID Q8N743

Separation of CD158z positive cells (red-filled) from CD158z negative cells (black-dashed) in flow cytometry analysis (surface staining) of KIR3DL3 (CD158z) transfected HEK-293 suspension stained using anti-human CD158z (CH21) PE antibody (concentration in sample 5 μg/ml).

Flow cytometry surface staining patterns of KIR3DL3 (CD158z) transfected HEK-293 suspension stained using anti-human CD158z (CH21) PE antibody (concentration in sample 5 μg/ml, left) or mouse IgG2a isotype control (MOPC-173) PE antibody (concentration in sample 5 μg/ml, same as CD158z PE concentration, right).

Product specific references:

Trundley AE, Hiby SE, Chang C, Sharkey AM, Santourlidis S, Uhrberg M, Trowsdale J, Moffett A: Molecular characterization of KIR3DL3. Immunogenetics 2006 Jan;57(12):904-16.
PubMed