Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD158z APC

Anti-Hu CD158z APC

Regulatory status

RUO

Antigen

CD158z

Clone

CH21

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

0.1 mg

1A-945-C100

In stock

220.00 USD

Variant

0.1 mg

1A-945-C100

In stock

220.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody CH21 recognizes an extracellular epitope of human CD158z (KIR3DL3), a transmembrane glycoprotein of killer cell inhibitory receptor family.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 3-12 µg/ml

Reactivity

Human

Immunogen

human CD158z transfectants

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

KIR3DL3

Antigen description

CD158z (KIR3DL3)is one of killer cell inhibitory receptors. It has three extracellular immunoglobulin-like domains and a long cytoplasmic tail, which, however, contains only one ITIM. Like other KIRs, CD158z is highly polymorphic, but it seems that its immunoglobulin-like domains are quite conserved among high primates.

Entrez Gene ID 115653

UniProt ID Q8N743

Flow cytometry surface staining pattern of KIR3DL3 (CD158z) transfected HEK-293 cells co-transfected with YFP coding plasmid using anti-human CD158z (CH21) APC antibody (concentration in sample 10 μg/ml).

Flow cytometry surface staining patterns of non-transfected HEK-293 cells and HEK-293 cells transfected with KIR-family coding plasmids co-transfected with YFP coding plasmid using anti-human CD158z (CH21) APC antibody (concentration in sample 10 μg/ml).

Product specific references:

Trundley AE, Hiby SE, Chang C, Sharkey AM, Santourlidis S, Uhrberg M, Trowsdale J, Moffett A: Molecular characterization of KIR3DL3. Immunogenetics 2006 Jan;57(12):904-16.
PubMed