Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD158f Alexa Fluor<sup>®</sup> 488

Anti-Hu CD158f Alexa Fluor^® 488

Regulatory status

RUO

Antigen

CD158f

Clone

UP-R1

Format

Alexa Fluor^® 488

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

A4-774-T100

In stock

248.60 USD

Variant

100 tests

A4-774-T100

In stock

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody UP-R1 recognizes an extracellular epitope on CD158f (KIR2DL5), a 60 kDa glycoprotein serving as a HLA class I ligand, and mainly expressed on a subset of NK cells and a small population of T cells. Its expression is highly polymorphic between individuals.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Human CD158f-Ig fusion protein

Preparation

Purified antibody is conjugated with Alexa Fluor^® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

KIR2DL5A

Antigen description

CD158f, also known as KIR2DL5, is a polymorphic 60 kDa transmembrane glycoprotein with two Ig-like extracellular domains by which it recognize HLA class I molecules. Its long intracellular domain contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that upon extracellular ligand-mediated phosphorylation serve as docking sites for inhibitory phosphatases, which results in blocking natural cytotoxicity as well as antibody-dependent cytotoxicity of the particular NK cell, and its adhesion toward target cells. Together with other killer inhibitory receptors CD158f is important for immunological tolerance to discriminate between normal and abnormal cells. Besides NK cells it is expressed on a small population of cytotoxic T cells. Expression of CD158f alleles is highly variable in the population.

Entrez Gene ID 57292

UniProt ID Q8N109

Separation of cells stained using anti-human CD158f (UP-R1) Alexa Fluor® 488 antibody (concentration in sample 1 μg/ml, red-filled) from cells using mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 488 antibody (concentration in sample 1 μg/ml, same as CD158f Alexa Fluor® 488 concentration, black-dashed) in flow cytometry analysis (surface staining) od KIR2DL5A transfected HEK-293 cell suspension.

General references:

Yusa S, Catina TL, Campbell KS: KIR2DL5 can inhibit human NK cell activation via recruitment of Src homology region 2-containing protein tyrosine phosphatase-2 (SHP-2). J Immunol. 2004 Jun 15;172(12):7385-92.
PubMed

Du Z, Sharma SK, Spellman S, Reed EF, Rajalingam R: KIR2DL5 alleles mark certain combination of activating KIR genes. Genes Immun. 2008 Jul;9(5):470-80.
PubMed

Product specific references:

Estefanía E, Flores R, Gómez-Lozano N, Aguilar H, López-Botet M, Vilches C: Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets. J Immunol. 2007 Apr 1;178(7):4402-10.
PubMed