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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD158d Purified Low Endotoxin
Anti-Hu CD158d Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD158d
Clone
mAb#33
Format
Purified Low Endotoxin
Reactivity
Human
Application
IP, WB, FC (QC tested), ICC, FUNC
Variant
0.1 mg
12-609-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Contact distributor
Product details
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d / KIR2DL4, a 45 kDa NK cell marker. Cell surface expression and function of CD158d / KIR2DL4 depends on genotype of particular individuals.
Application
IP, WB, FC (QC tested), ICC, FUNC
Application details
Flow cytometry: Recommended dilution: 2-6 µg/ml
Reactivity
Human
Immunogen
NK3.3 cells and KIR2DL4-Ig fusion protein
Other names
KIR2DL4, KIR103AS, 103AS, 15.212
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Product specific references:
Rajagopalan S, Bryceson YT, Kuppusamy SP, Geraghty DE, van der Meer A, Joosten I, Long EO. Activation of NK cells by an endocytosed receptor for soluble HLA-G. PLoS Biol. 2006 Jan;4(1):e9.
PubMed
Yan WH, Fan LA: Residues Met76 and Gln79 in HLA-G alpha1 domain involve in KIR2DL4 recognition. Cell Res. 2005 Mar;15(3):176-82.
PubMed
LeMaoult J, Zafaranloo K, Le Danff C, Carosella ED: HLA-G up-regulates ILT2, ILT3, ILT4, and KIR2DL4 in antigen presenting cells, NK cells, and T cells. FASEB J. 2005 Apr;19(6):662-4.
PubMed
Rajagopalan S, Fu J, Long EO: Cutting edge: induction of IFN-gamma production but not cytotoxicity by the killer cell Ig-like receptor KIR2DL4 (CD158d) in resting NK cells. J Immunol. 2001 Aug 15;167(4):1877-81.
PubMed
Goodridge JP, Witt CS, Christiansen FT, Warren HS: KIR2DL4 (CD158d) genotype influences expression and function in NK cells. J Immunol. 2003 Aug 15;171(4):1768-74.
PubMed
Variant
0.1 mg
12-609-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Contact distributor
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