Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD158d Purified Low Endotoxin

Anti-Hu CD158d Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD158d

Clone

mAb#33

Format

Purified Low Endotoxin

Reactivity

Human

Application

IP, WB, FC (QC tested), ICC, FUNC

Variant

0.1 mg

12-609-C100

In stock

180.00 USD

Variant

0.1 mg

12-609-C100

In stock

180.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d / KIR2DL4, a 45 kDa NK cell marker. Cell surface expression and function of CD158d / KIR2DL4 depends on genotype of particular individuals.

Application

IP, WB, FC (QC tested), ICC, FUNC

Application details

Flow cytometry: Recommended dilution: 2-6 µg/ml.
Functional application: Activation.

Reactivity

Human

Immunogen

NK3.3 cells and KIR2DL4-Ig fusion protein

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

KIR2DL4, KIR103AS, 103AS, 15.212

Antigen description

CD158d / KIR2DL4 is a KIR family member that shares structural features with both activating and inhibitory receptors and may mediate different functions under different circumstances. It contains cytoplasmic ITIM, suggesting inhibitory function, but also transmembrane domain similar to those of activating KIRs. It has been reported that CD158d serves as an inhibitory receptor for peripheral and uterine NK cells, but its ligation with soluble mAbs (unlike immobilized mAbs) results in activation of IFN-γ secretion. CD158d also binds both membrane form and soluble form of its ligand HLA-G.

Entrez Gene ID 3805

UniProt ID Q99706

Flow cytometry surface staining pattern of murine splenocytes stained using anti-human CD158d (mAb#33) purified antibody (concentration in sample 6 μg/ml, GAM APC).

Flow cytometry multicolor surface staining pattern of human CD3 negative lymphocytes using anti-human CD158d (mAb#33) purified antibody (concentration in sample 6 μg/ml, GAM APC) and anti-human CD56 (LT56) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD158d (mAb#33) purified antibody (concentration in sample 6 μg/ml, GAM APC) and anti-human CD3 (UCHT1) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD158d positive NK cells (red-filled) from T cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD158d (mAb#33) purified antibody (concentration in sample 6 μg/ml, GAM APC).

Product specific references:

Rajagopalan S, Bryceson YT, Kuppusamy SP, Geraghty DE, van der Meer A, Joosten I, Long EO. Activation of NK cells by an endocytosed receptor for soluble HLA-G. PLoS Biol. 2006 Jan;4(1):e9.
PubMed

Yan WH, Fan LA: Residues Met76 and Gln79 in HLA-G alpha1 domain involve in KIR2DL4 recognition. Cell Res. 2005 Mar;15(3):176-82.
PubMed

LeMaoult J, Zafaranloo K, Le Danff C, Carosella ED: HLA-G up-regulates ILT2, ILT3, ILT4, and KIR2DL4 in antigen presenting cells, NK cells, and T cells. FASEB J. 2005 Apr;19(6):662-4.
PubMed

Rajagopalan S, Fu J, Long EO: Cutting edge: induction of IFN-gamma production but not cytotoxicity by the killer cell Ig-like receptor KIR2DL4 (CD158d) in resting NK cells. J Immunol. 2001 Aug 15;167(4):1877-81.
PubMed

Goodridge JP, Witt CS, Christiansen FT, Warren HS: KIR2DL4 (CD158d) genotype influences expression and function in NK cells. J Immunol. 2003 Aug 15;171(4):1768-74.
PubMed