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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD158d Purified
Anti-Hu CD158d Purified
Regulatory status
RUO
Antigen
CD158d
Clone
mAb#33
Format
Purified
Reactivity
Human
Application
IP, FC (QC tested), ICC
Variant
0.1 mg
11-609-C100
In stock
198.00 USD
0.025 mg
11-609-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
11-609-C100
In stock
198.00 USD
0.025 mg
11-609-C025
Delivery 1 week
99.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d / KIR2DL4, a 45 kDa NK cell marker. Cell surface expression and function of CD158d / KIR2DL4 depends on genotype of particular individuals.
Application
IP, FC (QC tested), ICC
Application details
Flow cytometry: Recommended dilution: 2-6 μg/ml.
Reactivity
Human
Immunogen
NK3.3 cells and KIR2DL4-Ig fusion protein
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
KIR2DL4, KIR103AS, 103AS, 15.212
Antigen description
CD158d / KIR2DL4 is a KIR family member that shares structural features with both activating and inhibitory receptors and may mediate different functions under different circumstances. It contains cytoplasmic ITIM, suggesting inhibitory function, but also transmembrane domain similar to those of activating KIRs. It has been reported that CD158d serves as an inhibitory receptor for peripheral and uterine NK cells, but its ligation with soluble mAbs (unlike immobilized mAbs) results in activation of IFN-γ secretion. CD158d also binds both membrane form and soluble form of its ligand HLA-G.
Entrez Gene ID
3805
UniProt ID
Q99706
Flow cytometry multicolor surface staining of human lymphocytes using anti-human CD158d (mAb#33) purified antibody (concentration in sample 6,0 μg/ml, GAM APC) and surface anti-human CD56 (LT56) PE antibody (concentration in sample 1,0 μg/ml).
Flow cytometry multicolor surface staining pattern of human CD3 negative lymphocytes using anti-human CD158d (mAb#33) purified antibody (concentration in sample 6 μg/ml, GAM APC) and anti-human CD56 (LT56) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD158d (mAb#33) purified antibody (concentration in sample 6 μg/ml, GAM APC) and anti-human CD3 (UCHT1) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).
Separation of human CD158d positive CD56 positive CD3 negative NK cells (red-filled) from T cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD158d (mAb#33) purified antibody (concentration in sample 6 μg/ml, GAM APC).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD158d (mAb#33) purified antibody (concentration in sample 6 μg/ml, GAM APC).
Product specific references:
Rajagopalan S, Bryceson YT, Kuppusamy SP, Geraghty DE, van der Meer A, Joosten I, Long EO. Activation of NK cells by an endocytosed receptor for soluble HLA-G. PLoS Biol. 2006 Jan;4(1):e9.
PubMed
Yan WH, Fan LA: Residues Met76 and Gln79 in HLA-G alpha1 domain involve in KIR2DL4 recognition. Cell Res. 2005 Mar;15(3):176-82.
PubMed
LeMaoult J, Zafaranloo K, Le Danff C, Carosella ED: HLA-G up-regulates ILT2, ILT3, ILT4, and KIR2DL4 in antigen presenting cells, NK cells, and T cells. FASEB J. 2005 Apr;19(6):662-4.
PubMed
Rajagopalan S, Fu J, Long EO: Cutting edge: induction of IFN-gamma production but not cytotoxicity by the killer cell Ig-like receptor KIR2DL4 (CD158d) in resting NK cells. J Immunol. 2001 Aug 15;167(4):1877-81.
PubMed
Goodridge JP, Witt CS, Christiansen FT, Warren HS: KIR2DL4 (CD158d) genotype influences expression and function in NK cells. J Immunol. 2003 Aug 15;171(4):1768-74.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
11-609-C100
In stock
198.00 USD
0.025 mg
11-609-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
11-609-C100
In stock
198.00 USD
0.025 mg
11-609-C025
Delivery 1 week
99.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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