Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD158d APC

Anti-Hu CD158d APC

Regulatory status

RUO

Antigen

CD158d

Clone

mAb#33

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-609-T100

Ask for delivery term HERE

220.00 USD

25 tests

1A-609-T025

Delivery 1 week

110.00 USD

Variant

100 tests

1A-609-T100

Ask for delivery term HERE

220.00 USD

25 tests

1A-609-T025

Delivery 1 week

110.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d / KIR2DL4, a 45 kDa NK cell marker. Cell surface expression and function of CD158d / KIR2DL4 depends on genotype of particular individuals.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

NK3.3 cells and KIR2DL4-Ig fusion protein

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

KIR2DL4, KIR103AS, 103AS, 15.212

Antigen description

CD158d / KIR2DL4 is a KIR family member that shares structural features with both activating and inhibitory receptors and may mediate different functions under different circumstances. It contains cytoplasmic ITIM, suggesting inhibitory function, but also transmembrane domain similar to those of activating KIRs. It has been reported that CD158d serves as an inhibitory receptor for peripheral and uterine NK cells, but its ligation with soluble mAbs (unlike immobilized mAbs) results in activation of IFN-γ secretion. CD158d also binds both membrane form and soluble form of its ligand HLA-G.

Entrez Gene ID 3805

UniProt ID Q99706

Separation of cells stained using anti-human CD158d (mAb#33) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 6 μg/ml, same as CD158d APC concentration, black-dashed) in flow cytometry analysis (surface staining) of NKL cell suspension.

Flow cytometry analysis (surface staining) of CD158d in human peripheral blood using anti-CD158d (mAB#33) APC.

Product specific references:

Rajagopalan S, Bryceson YT, Kuppusamy SP, Geraghty DE, van der Meer A, Joosten I, Long EO. Activation of NK cells by an endocytosed receptor for soluble HLA-G. PLoS Biol. 2006 Jan;4(1):e9.
PubMed

Yan WH, Fan LA: Residues Met76 and Gln79 in HLA-G alpha1 domain involve in KIR2DL4 recognition. Cell Res. 2005 Mar;15(3):176-82.
PubMed

LeMaoult J, Zafaranloo K, Le Danff C, Carosella ED: HLA-G up-regulates ILT2, ILT3, ILT4, and KIR2DL4 in antigen presenting cells, NK cells, and T cells. FASEB J. 2005 Apr;19(6):662-4.
PubMed

Rajagopalan S, Fu J, Long EO: Cutting edge: induction of IFN-gamma production but not cytotoxicity by the killer cell Ig-like receptor KIR2DL4 (CD158d) in resting NK cells. J Immunol. 2001 Aug 15;167(4):1877-81.
PubMed

Goodridge JP, Witt CS, Christiansen FT, Warren HS: KIR2DL4 (CD158d) genotype influences expression and function in NK cells. J Immunol. 2003 Aug 15;171(4):1768-74.
PubMed