Anti-Hu CD158d Alexa Fluor® 647

Anti-Hu CD158d Alexa Fluor® 647
Regulatory status
RUO
Antigen
CD158d
Clone
mAb#33
Format
Alexa Fluor® 647
Reactivity
Human
Application
WB, FC (QC tested), ICC
Excitation laser
red (633 nm)
Variant
100 tests
A6-609-T100
Out of stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d / KIR2DL4, a 45 kDa NK cell marker. Cell surface expression and function of CD158d / KIR2DL4 depends on genotype of particular individuals.
Application
WB, FC (QC tested), ICC
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
NK3.3 cells and KIR2DL4-Ig fusion protein
Other names
KIR2DL4, KIR103AS, 103AS, 15.212
Preparation
The purified antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
A6-609_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD158d (mAb#33) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).
A6-609_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD158d (mAb#33) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD56 (LT56) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
A6-609_FC_Histogram
Separation of human CD158d positive CD56 positive NK cells (red-filled) from CD158d negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD158d (mAb#33) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Rajagopalan S, Bryceson YT, Kuppusamy SP, Geraghty DE, van der Meer A, Joosten I, Long EO. Activation of NK cells by an endocytosed receptor for soluble HLA-G. PLoS Biol. 2006 Jan;4(1):e9.
PubMed
Yan WH, Fan LA: Residues Met76 and Gln79 in HLA-G alpha1 domain involve in KIR2DL4 recognition. Cell Res. 2005 Mar;15(3):176-82.
PubMed
LeMaoult J, Zafaranloo K, Le Danff C, Carosella ED: HLA-G up-regulates ILT2, ILT3, ILT4, and KIR2DL4 in antigen presenting cells, NK cells, and T cells. FASEB J. 2005 Apr;19(6):662-4.
PubMed
Rajagopalan S, Fu J, Long EO: Cutting edge: induction of IFN-gamma production but not cytotoxicity by the killer cell Ig-like receptor KIR2DL4 (CD158d) in resting NK cells. J Immunol. 2001 Aug 15;167(4):1877-81.
PubMed
Goodridge JP, Witt CS, Christiansen FT, Warren HS: KIR2DL4 (CD158d) genotype influences expression and function in NK cells. J Immunol. 2003 Aug 15;171(4):1768-74.
PubMed
Variant
100 tests
A6-609-T100
Out of stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

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