Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD158agh Biotin

Anti-Hu CD158agh Biotin

Regulatory status

RUO

Antigen

CD158agh

Clone

HP-MA4

Format

Biotin

Reactivity

Human

Application

IP, FC (QC tested)

test1 True

Variant

0.1 mg

1B-775-C100

In stock

203.50 USD

Variant

0.1 mg

1B-775-C100

In stock

203.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody HP-MA4 recognizes an extracellular epitope of CD158 isoforms KIR2DL1 (CD158a), KIR2DS5 (CD158g), KIR2DS1 (CD158h), and KIR2DS3. It does not recognize the isoforms CD158b1,d,f,i,j.

Application

IP, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 0.5-4 μg/ml.

Reactivity

Human

Immunogen

Human NK cell line LB2

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

KIR2DL1, NKAT, KIR2DS1, KIR2DS3, NKAT7, KIR2DS5, NKAT9, CD158g, CD158h, CD158a

Antigen description

Killer cell immunoglobulin-like receptors (KIRs) are polymorphic transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. They are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain (such as CD158a / KIR2DL1) transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain (such as CD158g / KIR2DS5, CD158h / KIR2DS1, or KIR2DS3) lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for CD158 isoforms are subsets of MHC class I molecules.

Entrez Gene ID 3805

UniProt ID Q99706

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD158agh (HP-MA4) Biotin antibody (concentration in sample 1.67 μg/ml, Streptavidin APC).

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD158agh (HP-MA4) Biotin antibody (concentration in sample 1.67 μg/ml, Streptavidin APC) and anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD158agh positive CD3 negative lymphocytes (red-filled) from CD158agh negative CD3 positive T cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD158agh (HP-MA4) Biotin antibody (concentration in sample 1.67 μg/ml, Streptavidin APC).

Product specific references:

Wiernik A, Foley B, Zhang B, Verneris MR, Warlick E, Gleason MK, Ross JA, Luo X, Weisdorf DJ, Walcheck B, Vallera DA, Miller JS: Targeting natural killer cells to acute myeloid leukemia in vitro with a CD16 x 33 bispecific killer cell engager and ADAM17 inhibition. Clin Cancer Res. 2013 Jul 15;19(14):3844-55
PubMed