Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD156c APC

Anti-Hu CD156c APC

Regulatory status

RUO

Antigen

CD156c

Clone

11G2

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-938-T100

In stock

242.00 USD

Variant

100 tests

1A-938-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 11G2 recognizes an extracellular/luminal epitope of CD156c, a type I transmembrane glycoprotein, serving as a zinc-dependent metalloprotease.

Workshop

HCDM listed

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Negative species

Mouse

Immunogen

Jurkat cells

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ADAM10, AD10, RAK, MADM, HsT18717

Antigen description

CD156c is a type I transmembrane glycoprotein with a zinc-dependent metalloprotease activity. It serves as an endopeptidase of broad specificity, which is expressed mainly in thymus, liver, and muscles. Its expression can be induced in inflamed central nervous system, and in arthritic tissues. CD156c is involved in multiple sclerosis-associated myelin degradation. It also solubilizes various membrane proteins, including CD23, CD44, CD126, CD171, ephrin-A2, and other.

Entrez Gene ID 102

UniProt ID O14672

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD156c (11G2) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human monocytes (red-filled) from CD156c negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD156c (11G2) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Zola H et al.: Leukocyte and Stromal Cell Molecules, Wiley 2007

Arduise C, Abache T, Li L, Billard M, Chabanon A, Ludwig A, Mauduit P, Boucheix C, Rubinstein E, Le Naour F: Tetraspanins regulate ADAM10-mediated cleavage of TNF-alpha and epidermal growth factor. J Immunol. 2008 Nov 15;181(10):7002-13.
PubMed