Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD152 Purified Low Endotoxin

Anti-Hu CD152 Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD152

Clone

BNI3

Format

Purified Low Endotoxin

Reactivity

Human

Application

IP, FC (QC tested), IHC-F, ICC

Variant

0.1 mg

12-785-C100

In stock

143.00 USD

Variant

0.1 mg

12-785-C100

In stock

143.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody BNI3 recognizes an extracellular domain of human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.

Application

IP, FC (QC tested), IHC-F, ICC

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml, Intracellular staining.

Reactivity

Human

Immunogen

Human CD152-IgG heavy chain fusion protein

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CTLA4, GSE, GRD4

Antigen description

CD152 / CTLA-4 is a homodimeric transmembrane protein similar to CD28 and binding the same ligands, i.e. CD80 (B7.1) and CD86 (B7.2), but with higher affinity. Unlike CD28 with important costimulating functions, CD152 acts as an important inhibitory receptor essential for modulation of the immune system. CD152 / CTLA-4 becomes transiently expressed on activated T cells and its malfunction can cause autoimmune diseases, such as insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, or thyroid-associated orbitopathy.

Entrez Gene ID 1493

UniProt ID P16410

Flow cytometry surface staining pattern of human PHA stimulated peripheral whole blood stained using anti-human CD152 (BNI3) purified antibody (low endotoxin, concentration in sample 10 µg/ml) GAM APC.

Flow cytometry multicolor surface staining of human PHA stimulated lymphocytes stained using anti-human CD152 (BNI3) purified antibody (low endotoxin, concentration in sample 10 µg/ml, GAM APC) and anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD152 positive CD3 positive lymphocytes (red-filled) from CD152 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD152 (BNI3) purified antibody (low endotoxin, concentration in sample 10 µg/ml) GAM APC.

Product specific references:

Steiner K, Waase I, Rau T, Dietrich M, Fleischer B, Bröker BM: Enhanced expression of CTLA-4 (CD152) on CD4+ T cells in HIV infection. Clin Exp Immunol. 1999 Mar;115(3):451-7.
PubMed

Rissiek A, Baumann I, Cuapio A, Mautner A, Kolster M, Arck PC, Dodge-Khatami A, Mittrücker HW, Koch-Nolte F, Haag F, Tolosa E: The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation. J Autoimmun. 2015 Apr;58:12-20.
PubMed

Kraszula L, Eusebio M, Kupczyk M, Kuna P, Pietruczuk M: The use of multi-color flow cytometry for identification of functional markers of nTregs in patients with severe asthma. Pneumonol Alergol Pol. 2012;80(5):389-401.
PubMed

Chin LT, Chu C, Chen HM, Hsu SC, Weng BC, Chu CH: Site-directed in vitro immunization leads to a complete human monoclonal IgG4 lambda that binds specifically to the CDR2 region of CTLA-4 (CD152) without interfering the engagement of natural ligands. BMC Biotechnol. 2007 Aug 23;7:51.
PubMed

Steiner K, Moosig F, Csernok E, Selleng K, Gross WL, Fleischer B, Bröker BM: Increased expression of CTLA-4 (CD152) by T and B lymphocytes in Wegener's granulomatosis. Clin Exp Immunol. 2001 Oct;126(1):143-50.
PubMed